ArticleThe Journal of biological chemistry2008
Two stage cadherin kinetics require multiple extracellular domains but not the cytoplasmic region.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- Cadherins and growth factor receptors - ligand-selective mechano-switches at cadherin junctions.Journal of cell science · 2025Article
- Characterizing interactions in E-cadherin assemblages.Biophysical journal · 2023Article
- Engineering the Interactions of Classical Cadherin Cell-Cell Adhesion Proteins.Journal of immunology (Baltimore, Md. : 1950) · 2023Review
- A genetically encoded BRET-based SARS-CoV-2 MCommunications chemistry · 2022Article
- Computational model of E-cadherin clustering under force.Biophysical journal · 2021Article
- P120 catenin potentiates constitutive E-cadherin dimerization at the plasma membrane and regulates trans binding.Current biology : CB · 2021Article
- Article
- Cadherin clusters stabilized by a combination of specific and nonspecific cis-interactions.eLife · 2020Article
- Single-molecule studies of classical and desmosomal cadherin adhesion.Current opinion in biomedical engineering · 2019Article
- VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism.The Journal of cell biology · 2019Article
- A Computational Model for Kinetic Studies of Cadherin Binding and Clustering.Biophysical journal · 2016Article
- Binding equilibrium and kinetics of membrane-anchored receptors and ligands in cell adhesion: Insights from computational model systems and theory.Cell adhesion & migration · 2016Review
- Kinetic Measurements Reveal Enhanced Protein-Protein Interactions at Intercellular Junctions.Scientific reports · 2016Article
- Allosteric Regulation of E-Cadherin Adhesion.The Journal of biological chemistry · 2015Article
- Tissue mechanics and adhesion during embryo development.Developmental biology · 2015Review
- Linking 3D and 2D binding kinetics of membrane proteins by multiscale simulations.Protein science : a publication of the Protein Society · 2014Article
- α-catenin cytomechanics--role in cadherin-dependent adhesion and mechanotransduction.Journal of cell science · 2014Article
- Covalent and density-controlled surface immobilization of E-cadherin for adhesion force spectroscopy.PloS one · 2014Article
- Tuning the kinetics of cadherin adhesion.The Journal of investigative dermatology · 2013Review
- Ideal, catch, and slip bonds in cadherin adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Micropipette manipulation measurements quantified the pre-steady state binding kinetics between cell pairs mediated by Xenopus cleavage stage cadherin. The time-dependence of the intercellular binding probability exhibits a fast forming, low probability binding state, which transitions to a slower forming, high probability state. The biphasic kinetics are independent of the cytoplasmic region, but the transition to the high probability state requires the third extracellular domain EC3. Deleting either EC3 or EC3-5, or substituting Trp(2) for Ala reduces the binding curves to a simple, monophasic rise in binding probability to a limiting plateau, as predicted for a single site binding mechanism. The two stage cadherin binding process reported here directly parallels previous biophysical studies, and confirms that the cadherin ectodomain governs the initial intercellular adhesion dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.