ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2008
Immunohistochemical markers for quantitative studies of neurons and glia in human neocortex.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 2 of them syntheses that pooled it.
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Who cites it
49 citing papers in PubMed, 2 syntheses or guidelines pooled it, 98 citations in OpenAlex.
- Perfusion fixation in brain banking: a systematic review.Acta neuropathologica communications · 2019Pooled it
- Markers of microglia in post-mortem brain samples from patients with Alzheimer's disease: a systematic review.Molecular psychiatry · 2018Pooled it
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- Transcriptional evidence of reduced BDNF trophic capacity in the post-mortem human midbrain of schizophrenia cases with high inflammation.Translational psychiatry · 2025Article
- Spatiotemporal perturbations of the plasminogen activation system in a rat model of acute organophosphate intoxication.Acta neuropathologica communications · 2025Article
- Immunohistochemical field parcellation of the human hippocampus along its antero-posterior axis.Brain structure & function · 2024Article
- Differential effects of prolonged post-fixation on immunohistochemical and histochemical staining for postmortem human brains.Frontiers in neuroanatomy · 2024Article
- Fluid preservation in brain banking: a review.Free neuropathology · 2024Article
- A Standardized Protocol for the Safe Retrieval of Infectious Postmortem Human Brain for Studying Whole-Brain Pathology.The American journal of forensic medicine and pathology · 2023Article
- Neocortex neurogenesis and maturation in the African greater cane rat.Neural development · 2023Article
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- Sequences of synaptogenesis in the human fetal and neonatal brain by synaptophysin immunocytochemistry.Frontiers in cellular neuroscience · 2023Review
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- Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study.Frontiers in neuroanatomy · 2022Article
- Proceedings of the 2021 National Toxicology Program Satellite Symposium.Toxicologic pathology · 2021Article
- Cellular 3D-reconstruction and analysis in the human cerebral cortex using automatic serial sections.Communications biology · 2021Article
- Analysis of reactive astrogliosis in mouse brain usingSTAR protocols · 2021Article
- Developmental Differences in Neocortex Neurogenesis and Maturation Between the Altricial Dwarf Rabbit and Precocial Guinea Pig.Frontiers in neuroanatomy · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reproducible visualization of neurons and glia in human brain is essential for quantitative studies of the cellular changes in neurological disease. However, immunohistochemistry in human brain specimens is often compromised because of prolonged fixation. To select cell lineage-specific antibodies for quantitative studies of neurons and the major types of glia, we used 29 different antibodies, different epitope retrieval methods, and different detection systems to stain tissue arrays of formalin-fixed human brain. The screening pointed at CD45/leukocyte common antigen (LCA), CD68(KP1), 2',3' cyclic nucleotide phosphatase (CNPase), glial fibrillary acidic protein (GFAP), HLA-DR, Ki67, neuronal nuclei (NeuN), p25alpha-antigen, and S100beta as candidates for future cell counting purposes, because these markers visualized specific neuronal and glial cell bodies. However, significant negative correlation between staining result and formalin fixation was observed by blinded scoring of staining for CD45/LCA, CNPase, GFAP, and NeuN in brain specimens fixed by immersion and stored up to 10 years in 4% formalin solution at room temperature, independent of donor sex and postmortem interval. In contrast, improved preservation of NeuN and CNPase staining, and full preservation of GFAP and CD45/LCA staining in tissue fixed by perfusion and stored for up to 3 years in 0.1% paraformaldehyde solution at 4C, indicated that immunohistochemistry can be performed in well-preserved biobank material.
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