ArticleNucleic acids research2007
A ligation assay for multiplex analysis of CpG methylation using bisulfite-treated DNA.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Obesity Inhibits Angiogenesis Through TWIST1-SLIT2 Signaling.Frontiers in cell and developmental biology · 2021Article
- Reevaluation ofFrontiers in molecular neuroscience · 2018Review
- A Set of Assays for the Comprehensive Analysis of FMR1 Alleles in the Fragile X-Related Disorders.The Journal of molecular diagnostics : JMD · 2016Article
- Novel multiplex MethyLight protocol for detection of DNA methylation in patient tissues and bodily fluids.Scientific reports · 2014Article
- A novel methylation PCR that offers standardized determination of FMR1 methylation and CGG repeat length without southern blot analysis.The Journal of molecular diagnostics : JMD · 2014Article
- Methylation-specific ligation detection reaction (msLDR): a new approach for multiplex evaluation of methylation patterns.Molecular genetics and genomics : MGG · 2011Article
- FMR1 intron 1 methylation predicts FMRP expression in blood of female carriers of expanded FMR1 alleles.The Journal of molecular diagnostics : JMD · 2011Article
- High-resolution methylation polymerase chain reaction for fragile X analysis: evidence for novel FMR1 methylation patterns undetected in Southern blot analyses.Genetics in medicine : official journal of the American College of Medical Genetics · 2011Article
- Custom-designed MLPA using multiple short synthetic probes: application to methylation analysis of five promoter CpG islands in tumor and urine specimens from patients with bladder cancer.The Journal of molecular diagnostics : JMD · 2010Article
- Methylated APC and GSTP1 genes in serum DNA correlate with the presence of circulating blood tumor cells and are associated with a more aggressive and advanced breast cancer disease.European journal of medical research · 2010Article
- Similarity of aberrant DNA methylation in Barrett's esophagus and esophageal adenocarcinoma.Molecular cancer · 2008Article
- Sensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aberrant methylation of promoter CpG islands is causally linked with a number of inherited syndromes and most sporadic cancers, and may provide valuable diagnostic and prognostic biomarkers. In this report, we describe an approach to simultaneous analysis of multiple CpG islands, where methylation-specific oligonucleotide probes are joined by ligation and subsequently amplified by polymerase chain reaction (PCR) when hybridized in juxtaposition on bisulfite-treated DNA. Specificity of the ligation reaction is achieved by (i) using probes containing CpGpCpG (for methylated sequences) or CpApCpA (for unmethylated sequences) at the 3' ends, (ii) including three or more probes for each target, and (iii) using a thermostable DNA ligase. The external probes carry universal tails to allow amplification of multiple ligation products using a common primer pair. As proof-of-principle applications, we established duplex assays to examine the FMR1 promoter in individuals with fragile-X syndrome and the SNRPN promoter in individuals with Prader-Willi syndrome or Angelman syndrome, and a multiplex assay to simultaneously detect hypermethylation of seven genes (ID4, APC, RASSF1A, CDH1, ESR1, HIN1 and TWIST1) in breast cancer cell lines and tissues. These data show that ligation of oligonucleotide probes hybridized to bisulfite-treated DNA is a simple and cost-effective approach to analysis of CpG methylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.