ArticleVirology2008
Analysis of human immunodeficiency virus matrix domain replacements.
Article in Virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Evasion of CARD8 activation during HIV-1 assembly.Science advances · 2025Article
- Article
- Review
- Article
- Trimer Enhancement Mutation Effects on HIV-1 Matrix Protein Binding Activities.Journal of virology · 2016Article
- Analysis of HIV-1 Gag protein interactions via biotin ligase tagging.Journal of virology · 2015Article
- Article
- Diverse viral glycoproteins as well as CD4 co-package into the same human immunodeficiency virus (HIV-1) particles.Retrovirology · 2014Article
- The roles of lipids and nucleic acids in HIV-1 assembly.Frontiers in microbiology · 2014Review
- Second site reversion of a mutation near the amino terminus of the HIV-1 capsid protein.Virology · 2013Article
- Multiple Gag domains contribute to selective recruitment of murine leukemia virus (MLV) Env to MLV virions.Journal of virology · 2013Article
- Analysis of small molecule ligands targeting the HIV-1 matrix protein-RNA binding site.The Journal of biological chemistry · 2013Article
- Cytoplasmic utilization of human immunodeficiency virus type 1 genomic RNA is not dependent on a nuclear interaction with gag.Journal of virology · 2012Article
- Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membrane.Journal of virology · 2011Article
- Rous sarcoma virus gag has no specific requirement for phosphatidylinositol-(4,5)-bisphosphate for plasma membrane association in vivo or for liposome interaction in vitro.Journal of virology · 2011Article
- HIV-1 matrix protein binding to RNA.Journal of molecular biology · 2011Article
- Second-site compensatory mutations of HIV-1 capsid mutations.Journal of virology · 2011Article
- Analysis of the N-terminal region of the murine leukemia virus nucleocapsid protein.Virus research · 2011Article
- Analysis of human immunodeficiency virus type 1 matrix binding to membranes and nucleic acids.Journal of virology · 2009Article
- HIV-1 matrix organizes as a hexamer of trimers on membranes containing phosphatidylinositol-(4,5)-bisphosphate.Virology · 2009Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The matrix (MA) domain of the HIV-1 structural precursor Gag (PrGag) protein targets PrGag proteins to membrane assembly sites, and facilitates incorporation of envelope proteins into virions. To evaluate the specific requirements for the MA membrane-binding domain (MBD) in HIV-1 assembly and replication, we examined viruses in which MA was replaced by alternative MBDs. Results demonstrated that the pleckstrin homology domains of AKT protein kinase and phospholipase C delta1 efficiently directed the assembly and release of virus-like particles (VLPs) from cells expressing chimeric proteins. VLP assembly and release also were mediated in a phorbol ester-dependent fashion by the cysteine-rich binding domain of phosphokinase Cgamma. Although alternative MBDs promoted VLP assembly and release, the viruses were not infectious. Notably, PrGag processing was reduced, while cleavage of GagPol precursors resulted in the accumulation of Pol-derived intermediates within virions. Our results indicate that the HIV-1 assembly machinery is flexible with regard to its means of membrane association, but that alternative MBDs can interfere with the elaboration of infectious virus cores.
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Registered trials
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