ArticleBiology of the cell2008
A transient forward-targeting element for microneme-regulated secretion in Toxoplasma gondii.
Article in Biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Article
- Toxoplasma FER1 is a versatile and dynamic mediator of differential microneme trafficking and microneme exocytosis.Scientific reports · 2024Article
- Article
- A cathepsin C-like protease post-translationally modifiesbioRxiv : the preprint server for biology · 2023Article
- An image-based high-content screening for compounds targetingFrontiers in cellular and infection microbiology · 2023Article
- Calcium negatively regulates secretion from dense granules in Toxoplasma gondii.Cellular microbiology · 2019Article
- Plasmodium falciparum dipeptidyl aminopeptidase 3 activity is important for efficient erythrocyte invasion by the malaria parasite.PLoS pathogens · 2018Article
- Intersection of endocytic and exocytic systems in Toxoplasma gondii.Traffic (Copenhagen, Denmark) · 2018Article
- Article
- Article
- A Critical Role for Toxoplasma gondii Vacuolar Protein Sorting VPS9 in Secretory Organelle Biogenesis and Host Infection.Scientific reports · 2016Article
- Unconventional endosome-like compartment and retromer complex in Toxoplasma gondii govern parasite integrity and host infection.Nature communications · 2016Article
- Protein trafficking through the endosomal system prepares intracellular parasites for a home invasion.PLoS pathogens · 2013Review
- Non-canonical maturation of two papain-family proteases in Toxoplasma gondii.The Journal of biological chemistry · 2013Article
- Forward targeting of Toxoplasma gondii proproteins to the micronemes involves conserved aliphatic amino acids.Traffic (Copenhagen, Denmark) · 2011Article
- Cathepsin proteases in Toxoplasma gondii.Advances in experimental medicine and biology · 2011Review
- Cell cycle-dependent, intercellular transmission of Toxoplasma gondii is accompanied by marked changes in parasite gene expression.Molecular microbiology · 2011Article
- Toxoplasma gondii transmembrane microneme proteins and their modular design.Molecular microbiology · 2010Article
- Cathepsin L occupies a vacuolar compartment and is a protein maturase within the endo/exocytic system of Toxoplasma gondii.Molecular microbiology · 2010Article
- Genetic evidence that an endosymbiont-derived endoplasmic reticulum-associated protein degradation (ERAD) system functions in import of apicoplast proteins.The Journal of biological chemistry · 2009Article
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Authors and funding
5 authors.
Funding
Abstract
background informationAccurate sorting of proteins to the three types of secretory granules in Toxoplasma gondii is crucial for successful cell invasion by this obligate intracellular parasite. As in other eukaryotic systems, propeptide sequences are a common yet poorly understood feature of proteins destined for regulated secretion, which for Toxoplasma occurs through two distinct invasion organelles, rhoptries and micronemes. Microneme discharge during parasite apical attachment plays a pivotal role in cell invasion by delivering adhesive proteins for host receptor engagement.
resultsWe show here that the small micronemal proprotein MIC5 (microneme protein-5) undergoes proteolytic maturation at a site beyond the Golgi, and only the processed form of MIC5 is secreted via the micronemes. Proper cleavage of the MIC5 propeptide relies on an arginine residue in the P1' position, although P1' mutants are still cleaved to a lesser extent at an alternative site downstream of the primary site. Nonetheless, this aberrantly cleaved species still correctly traffics to the micronemes, indicating that correct cleavage is not necessary for micronemal targeting. In contrast, a deletion mutant lacking the propeptide was retained within the secretory system, principally in the ER (endoplasmic reticulum). The MIC5 propeptide also supported correct trafficking when exchanged for the M2AP propeptide, which was recently shown to also be required for micronemal trafficking of the TgMIC2 (T. gondii MIC2)-M2AP complex [Harper, Huynh, Coppens, Parussini, Moreno and Carruthers (2006) Mol. Biol. Cell 17, 4551-4563].
conclusionOur results illuminate common and unique features of micronemal propeptides in their role as trafficking facilitators.
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