ArticlePlant physiology2008
The catalytic properties of hybrid Rubisco comprising tobacco small and sunflower large subunits mirror the kinetically equivalent source Rubiscos and can support tobacco growth.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 131 citations in OpenAlex.
- Laboratory evolution of Rubisco solubility and catalytic switches to enhance plant productivity.Nature plants · 2025Article
- Review
- Review
- Adapting CProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Increasing Rubisco as a simple means to enhance photosynthesis and productivity now without lowering nitrogen use efficiency.The New phytologist · 2025Review
- Genome-Wide Analysis and Expression Profiling of SoybeanInternational journal of molecular sciences · 2024Article
- Improving Crop Yield through Increasing Carbon Gain and Reducing Carbon Loss.Plants (Basel, Switzerland) · 2024Review
- The role of SWEET4 proteins in the post-phloem sugar transport pathway of Setaria viridis sink tissues.Journal of experimental botany · 2023Article
- Temperature sensitivity of carbon concentrating mechanisms in the diatom Phaeodactylum tricornutum.Photosynthesis research · 2023Article
- The trajectory in catalytic evolution of Rubisco in Posidonia seagrass species differs from terrestrial plants.Plant physiology · 2023Article
- New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields.Plant physiology · 2022Review
- Correlative adaptation between Rubisco and CONature plants · 2022Article
- Green giant-a tiny chloroplast genome with mighty power to produce high-value proteins: history and phylogeny.Plant biotechnology journal · 2021Review
- Bundle sheath suberisation is required for CCommunications biology · 2021Article
- The dependency of red Rubisco on its cognate activase for enhancing plant photosynthesis and growth.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Measuring Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays.Journal of experimental botany · 2020Article
- Modifying Plant Photosynthesis and Growth via Simultaneous Chloroplast Transformation of Rubisco Large and Small Subunits.The Plant cell · 2020Article
- Overexpression of BUNDLE SHEATH DEFECTIVE 2 improves the efficiency of photosynthesis and growth in Arabidopsis.The Plant journal : for cell and molecular biology · 2020Article
- Oxygen response of leaf COJournal of plant research · 2020Article
- Hybrid Cyanobacterial-Tobacco Rubisco Supports Autotrophic Growth and Procarboxysomal Aggregation.Plant physiology · 2020Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plastomic replacement of the tobacco (Nicotiana tabacum) Rubisco large subunit gene (rbcL) with that from sunflower (Helianthus annuus; rbcL(S)) produced tobacco(Rst) transformants that produced a hybrid Rubisco consisting of sunflower large and tobacco small subunits (L(s)S(t)). The tobacco(Rst) plants required CO(2) (0.5% v/v) supplementation to grow autotrophically from seed despite the substrate saturated carboxylation rate, K(m), for CO(2) and CO(2)/O(2) selectivity of the L(s)S(t) enzyme mirroring the kinetically equivalent tobacco and sunflower Rubiscos. Consequently, at the onset of exponential growth when the source strength and leaf L(s)S(t) content were sufficient, tobacco(Rst) plants grew to maturity without CO(2) supplementation. When grown under a high pCO(2), the tobacco(Rst) seedlings grew slower than tobacco and exhibited unique growth phenotypes: Juvenile plants formed clusters of 10 to 20 structurally simple oblanceolate leaves, developed multiple apical meristems, and the mature leaves displayed marginal curling and dimpling. Depending on developmental stage, the L(s)S(t) content in tobacco(Rst) leaves was 4- to 7-fold less than tobacco, and gas exchange coupled with chlorophyll fluorescence showed that at 2 mbar pCO(2) and growth illumination CO(2) assimilation in mature tobacco(Rst) leaves remained limited by Rubisco activity and its rate (approximately 11 micromol m(-2) s(-1)) was half that of tobacco controls. (35)S-methionine labeling showed the stability of assembled L(s)S(t) was similar to tobacco Rubisco and measurements of light transient CO(2) assimilation rates showed L(s)S(t) was adequately regulated by tobacco Rubisco activase. We conclude limitations to tobacco(Rst) growth primarily stem from reduced rbcL(S) mRNA levels and the translation and/or assembly of sunflower large with the tobacco small subunits that restricted L(s)S(t) synthesis.
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