ArticlePlant physiology2008
A versatile transposon-based activation tag vector system for functional genomics in cereals and other monocot plants.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 75 citations in OpenAlex.
- Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics.Plants (Basel, Switzerland) · 2025Review
- Development of Ac- and Ds-tagged starter lines for large-scale transposon-mutagenesis in tomato.PloS one · 2025Article
- Development of activation-tagged gain-of-functional mutants in indica rice line (BPT 5204) for sheath blight resistance.Molecular biology reports · 2024Article
- A BTB-TAZ protein is required for gene activation by Cauliflower mosaic virus 35S multimerized enhancers.Plant physiology · 2022Article
- Gain-of-function mutagenesis through activation tagging identifies XPB2 and SEN1 helicase genes as potential targets for drought stress tolerance in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Development ofPlant direct · 2021Article
- Genetic Diversity of Selected Rice Genotypes under Water Stress Conditions.Plants (Basel, Switzerland) · 2020Article
- An Efficient System forPlant physiology · 2019Article
- Co-culturing on dry filter paper significantly increased the efficiency ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Development of an activation tagging system for maize.Plant direct · 2019Article
- A Versatile Vector Toolkit for Functional Analysis of Rice Genes.Rice (New York, N.Y.) · 2018Article
- Activation-tagging in indica rice identifies a novel transcription factor subunit, NF-YC13 associated with salt tolerance.Scientific reports · 2017Article
- Gene Overexpression Resources in Cereals for Functional Genomics and Discovery of Useful Genes.Frontiers in plant science · 2016Review
- Efficient generation of marker-free transgenic rice plants using an improved transposon-mediated transgene reintegration strategy.Plant physiology · 2015Article
- Identification and use of the sugarcane bacilliform virus enhancer in transgenic maize.BMC plant biology · 2014Article
- Prokaryotic expression and purification of soluble maize Ac transposase.Molecular biotechnology · 2013Article
- An active ac/ds transposon system for activation tagging in tomato cultivar m82 using clonal propagation.Plant physiology · 2013Article
- Article
- A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery.BMC genomics · 2012Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transposon insertional mutagenesis is an effective alternative to T-DNA mutagenesis when transformation through tissue culture is inefficient as is the case for many crop species. When used as activation tags, transposons can be exploited to generate novel gain-of-function phenotypes without transformation and are of particular value in the study of polyploid plants where gene knockouts will not have phenotypes. We have developed an in cis-activation-tagging Ac-Ds transposon system in which a T-DNA vector carries a Dissociation (Ds) element containing 4x cauliflower mosaic virus enhancers along with the Activator (Ac) transposase gene. Stable Ds insertions were selected using green fluorescent protein and red fluorescent protein genes driven by promoters that are functional in maize (Zea mays) and rice (Oryza sativa). The system has been tested in rice, where 638 stable Ds insertions were selected from an initial set of 26 primary transformants. By analysis of 311 flanking sequences mapped to the rice genome, we could demonstrate the wide distribution of the elements over the rice chromosomes. Enhanced expression of rice genes adjacent to Ds insertions was detected in the insertion lines using semiquantitative reverse transcription-PCR method. The in cis-two-element vector system requires minimal number of primary transformants and eliminates the need for crossing, while the use of fluorescent markers instead of antibiotic or herbicide resistance increases the applicability to other plants and eliminates problems with escapes. Because Ac-Ds has been shown to transpose widely in the plant kingdom, the activation vector system developed in this study should be of utility more generally to other monocots.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.