ArticleJournal of bacteriology2008
Transcriptome analysis of a phenol-producing Pseudomonas putida S12 construct: genetic and physiological basis for improved production.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 63 citations in OpenAlex.
- EngineeringMetabolic engineering communications · 2026Article
- Two-Omics Probe on the Potential of Pseudomonas sp. GDMCC 1.1703 Under Phenol Stress.Current microbiology · 2023Article
- Engineering a Pseudomonas taiwanensis 4-coumarate platform for production of para-hydroxy aromatics with high yield and specificity.Metabolic engineering · 2023Article
- Insights into the susceptibility of Pseudomonas putida to industrially relevant aromatic hydrocarbons that it can synthesize from sugars.Microbial cell factories · 2023Article
- Rational Engineering of Phenylalanine Accumulation inFrontiers in bioengineering and biotechnology · 2019Article
- High-Yield Production of 4-Hydroxybenzoate From Glucose or Glycerol by an EngineeredFrontiers in bioengineering and biotechnology · 2019Article
- Protective role of glycerol against benzene stress: insights from the Pseudomonas putida proteome.Current genetics · 2016Article
- Metabolic Engineering of Pseudomonas putida KT2440 to Produce Anthranilate from Glucose.Frontiers in microbiology · 2015Article
- Metabolic engineering of Pseudomonas sp. strain VLB120 as platform biocatalyst for the production of isobutyric acid and other secondary metabolites.Microbial cell factories · 2014Article
- Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome.Microbial biotechnology · 2013Article
- p-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans.Microbial cell factories · 2013Article
- Systems metabolic engineering of microorganisms for natural and non-natural chemicals.Nature chemical biology · 2012Review
- Metabolic and regulatory rearrangements underlying efficient D-xylose utilization in engineered Pseudomonas putida S12.The Journal of biological chemistry · 2012Article
- Comparative transcriptomics and proteomics of p-hydroxybenzoate producing Pseudomonas putida S12: novel responses and implications for strain improvement.Applied microbiology and biotechnology · 2010Article
- TrgI, toluene repressed gene I, a novel gene involved in toluene-tolerance in Pseudomonas putida S12.Extremophiles : life under extreme conditions · 2009Article
- Bioproduction of p-hydroxystyrene from glucose by the solvent-tolerant bacterium Pseudomonas putida S12 in a two-phase water-decanol fermentation.Applied and environmental microbiology · 2009Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unknown genetic basis for improved phenol production by a recombinant Pseudomonas putida S12 derivative bearing the tpl (tyrosine-phenol lyase) gene was investigated via comparative transcriptomics, nucleotide sequence analysis, and targeted gene disruption. We show upregulation of tyrosine biosynthetic genes and possibly decreased biosynthesis of tryptophan caused by a mutation in the trpE gene as the genetic basis for the enhanced phenol production. In addition, several genes in degradation routes connected to the tyrosine biosynthetic pathway were upregulated. This either may be a side effect that negatively affects phenol production or may point to intracellular accumulation of tyrosine or its intermediates. A number of genes identified by the transcriptome analysis were selected for targeted disruption in P. putida S12TPL3. Physiological and biochemical examination of P. putida S12TPL3 and these mutants led to the conclusion that the metabolic flux toward tyrosine in P. putida S12TPL3 was improved to such an extent that the heterologous tyrosine-phenol lyase enzyme had become the rate-limiting step in phenol biosynthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.