ArticleJournal of bacteriology2008
Conditional lethality, division defects, membrane involution, and endocytosis in mre and mrd shape mutants of Escherichia coli.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers.
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Who cites it
140 citing papers in PubMed.
- The phosphoenolpyruvate phosphotransferase system regulates cell length and width through semi-independent pathways.Molecular biology of the cell · 2026Article
- Direct interaction between MreB and the RodA-PBP2 complex organizes lateral cell wall synthesis inmLife · 2026Article
- How Salmonella Works Under Osmotic and Desiccation Stresses.Comprehensive reviews in food science and food safety · 2026Review
- SanA Plays a Role in Peptidoglycan Integrity in Escherichia coli.Molecular microbiology · 2026Article
- Alanine-scanning mutagenesis library of MreB reveals distinct roles for regulating cell shape and viability.PLoS genetics · 2026Article
- Modulation of bacterial cell size and growth rate via activation of a cell envelope stress response.mBio · 2025Article
- Conformational regulation of two essential activators of bacterial cell elongation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Development of Broad-Spectrum Antimicrobial Peptides through the Conjugation of FtsZ-Binding and Cell-Penetrating Peptides.ACS infectious diseases · 2025Article
- Evolutionary rescue of sphericaleLife · 2025Article
- Characterization of MreCD inJournal of oral microbiology · 2025Article
- Verrucomicrobia of the FamilyMicroorganisms · 2024Article
- MreC-MreD structure reveals a multifaceted interface that controls MreC conformation.bioRxiv : the preprint server for biology · 2024Article
- Impact of AbaI mutation on virulence, biofilm development, and antibiotic susceptibility in Acinetobacter baumannii.Scientific reports · 2024Article
- The HtrA chaperone monitors sortase-assembled pilus biogenesis in Enterococcus faecalis.PLoS genetics · 2024Article
- Reply to Hong et al.: Synthetic lethality ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Chemical genetic approaches for the discovery of bacterial cell wall inhibitors.RSC medicinal chemistry · 2023Review
- Relationship between the Rod complex and peptidoglycan structure in Escherichia coli.MicrobiologyOpen · 2023Article
- Evidence of two differentially regulated elongasomes in Salmonella.Communications biology · 2023Article
- A role for the Gram-negative outer membrane in bacterial shape determination.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Unique N-Terminal Domain of Chlamydial Bactofilin Mediates Its Membrane Localization and Ring-Forming Properties.Journal of bacteriology · 2023Article
80 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
Abstract
Maintenance of rod shape in Escherichia coli requires the shape proteins MreB, MreC, MreD, MrdA (PBP2), and MrdB (RodA). How loss of the Mre proteins affects E. coli viability has been unclear. We generated Mre and Mrd depletion strains under conditions that minimize selective pressure for undefined suppressors and found their phenotypes to be very similar. Cells lacking one or more of the five proteins were fully viable and propagated as small spheres under conditions of slow mass increase but formed large nondividing spheroids with noncanonical FtsZ assembly patterns at higher mass doubling rates. Extra FtsZ was sufficient to suppress lethality in each case, allowing cells to propagate as small spheres under any condition. The failure of each unsuppressed mutant to divide under nonpermissive conditions correlated with the presence of elaborate intracytoplasmic membrane-bound compartments, including vesicles/vacuoles and more-complex systems. Many, if not all, of these compartments formed by FtsZ-independent involution of the cytoplasmic membrane (CM) rather than de novo. Remarkably, while some of the compartments were still continuous with the CM and the periplasm, many were topologically separate, indicating they had been released into the cytoplasm by an endocytic-like membrane fission event. Notably, cells failed to adjust the rate of phospholipid synthesis to their new surface requirements upon depletion of MreBCD, providing a rationale for the "excess" membrane in the resulting spheroids. Both FtsZ and MinD readily assembled on intracytoplasmic membrane surfaces, and we propose that this contributes significantly to the lethal division block seen in all shape mutants under nonpermissive conditions.
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