ArticleJournal of bacteriology2008
Multiple phospholipid N-methyltransferases with distinct substrate specificities are encoded in Bradyrhizobium japonicum.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Structural basis for phosphatidylcholine synthesis by bacterial phospholipid N-methyltransferases.The Journal of biological chemistry · 2025Article
- Three separate pathways inApplied and environmental microbiology · 2024Article
- Analysis of Bacterial Phosphorylcholine-Related Genes Reveals an Association between Type-Specific Biosynthesis Pathways and Biomolecules Targeted for Phosphorylcholine Modification.Microbiology spectrum · 2023Article
- Recombinant and endogenous ways to produce methylated phospholipids in Escherichia coli.Applied microbiology and biotechnology · 2021Article
- PhospholipidApplied and environmental microbiology · 2021Article
- The PhospholipidFrontiers in microbiology · 2021Article
- 1,2-Diacylglycerol choline phosphotransferase catalyzes the final step in the unique Treponema denticola phosphatidylcholine biosynthesis pathway.Molecular microbiology · 2017Article
- OlsG (Sinac_1600) Is an Ornithine Lipid N-Methyltransferase from the Planctomycete Singulisphaera acidiphila.The Journal of biological chemistry · 2015Article
- Membrane lipids in Agrobacterium tumefaciens: biosynthetic pathways and importance for pathogenesis.Frontiers in plant science · 2014Review
- S-adenosylmethionine-binding properties of a bacterial phospholipid N-methyltransferase.Journal of bacteriology · 2011Article
- Proteomic and transcriptomic characterization of a virulence-deficient phosphatidylcholine-negative Agrobacterium tumefaciens mutant.Molecular genetics and genomics : MGG · 2010Article
- In vitro characterization of the enzyme properties of the phospholipid N-methyltransferase PmtA from Agrobacterium tumefaciens.Journal of bacteriology · 2009Article
- Expression and physiological relevance of Agrobacterium tumefaciens phosphatidylcholine biosynthesis genes.Journal of bacteriology · 2009Article
- CTP synthetase and its role in phospholipid synthesis in the yeast Saccharomyces cerevisiae.Progress in lipid research · 2008Review
- Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum.Molecular genetics and genomics : MGG · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylcholine (PC) is the major phospholipid in eukaryotic membranes. In contrast, it is found in only a few prokaryotes including members of the family Rhizobiaceae. In these bacteria, PC is required for pathogenic and symbiotic plant-microbe interactions, as shown for Agrobacterium tumefaciens and Bradyrhizobium japonicum. At least two different phospholipid N-methyltransferases (PmtA and PmtX) have been postulated to convert phosphatidylethanolamine (PE) to PC in B. japonicum by three consecutive methylation reactions. However, apart from the known PmtA enzyme, we identified and characterized three additional pmt genes (pmtX1, pmtX3, and pmtX4), which can be functionally expressed in Escherichia coli, showing different substrate specificities. B. japonicum expressed only two of these pmt genes (pmtA and pmtX1) under all conditions tested. PmtA predominantly converts PE to monomethyl PE, whereas PmtX1 carries out both subsequent methylation steps. B. japonicum is the first bacterium known to use two functionally different Pmts. It also expresses a PC synthase, which produces PC via condensation of CDP-diacylglycerol and choline. Our study shows that PC biosynthesis in bacteria can be much more complex than previously anticipated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.