ArticleBiophysical journal2008
Stochastic study of the effect of ionic strength on noncovalent interactions in protein pores.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
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- Nanopore-based detection of periodontitis biomarker miR31 in saliva samples.Electrophoresis · 2024Article
- Probe-assisted detection of FeBiosensors & bioelectronics · 2024Article
- Quantification of Protein Glycosylation Using Nanopores.Nano letters · 2022Article
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- Nanopore detection of metal ions: Current status and future directions.Small methods · 2020Article
- Single-molecule Study on the Interactions between Cyclic Nonribosomal Peptides and Protein Nanopore.ACS applied bio materials · 2020Article
- Enzymatic reaction-based nanopore detection of zinc ions.The Analyst · 2019Article
- Salt-Mediated Nanopore Detection of ADAM-17.ACS applied bio materials · 2019Article
- Computation-Assisted Nanopore Detection of Thorium Ions.Analytical chemistry · 2018Article
- Peptide-Mediated Nanopore Detection of Uranyl Ions in Aqueous Media.ACS sensors · 2017Article
- Role of Cholesterol and Phospholipids in Amylin Misfolding, Aggregation and Etiology of Islet Amyloidosis.Advances in experimental medicine and biology · 2015Review
- Real-time label-free measurement of HIV-1 protease activity by nanopore analysis.Biosensors & bioelectronics · 2014Article
- Nanopore biosensor for label-free and real-time detection of anthrax lethal factor.ACS applied materials & interfaces · 2014Article
- Nanopore detection of copper ions using a polyhistidine probe.Biosensors & bioelectronics · 2014Article
- Translocation of single-stranded DNA through the α-hemolysin protein nanopore in acidic solutions.Electrophoresis · 2011Article
- Hofmeister effect in confined spaces: halogen ions and single molecule detection.Biophysical journal · 2011Article
- Unzipping of Double-stranded DNA in Engineered α-Hemolysin Pores.The journal of physical chemistry letters · 2011Article
- Ion selectivity of alpha-hemolysin with beta-cyclodextrin adapter. II. Multi-ion effects studied with grand canonical Monte Carlo/Brownian dynamics simulations.The journal of physical chemistry. B · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salt plays a critical role in the physiological activities of cells. We show that ionic strength significantly affects the kinetics of noncovalent interactions in protein channels, as observed in stochastic studies of the transfer of various analytes through pores of wild-type and mutant alpha-hemolysin proteins. As the ionic strength increased, the association rate constant of electrostatic interactions was accelerated, whereas those of both hydrophobic and aromatic interactions were retarded. Dramatic decreases in the dissociation rate constants, and thus increases in the overall reaction formation constants, were observed for all noncovalent interactions studied. The results suggest that with the increase of salt concentration, the streaming potentials for all the protein pores decrease, whereas the preferential selectivities of the pores for either cations or anions drop. Furthermore, results also show that the salt effect on the rate of association of analytes to a pore differs significantly depending on the nature of the noncovalent interactions occurring in the protein channel. In addition to providing new insights into the nature of analyte-protein pore interactions, the salt-dependence of noncovalent interactions in protein pores observed provides a useful means to greatly enhance the sensitivity of the nanopore, which may find useful application in stochastic sensing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.