ArticleMolecular and cellular biology2008
Naturally extended CT . AG repeats increase H-DNA structures and promoter activity in the smooth muscle myosin light chain kinase gene.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Triple-helix potential of the mouse genome.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Effect of dC → d(mBiochimie · 2018Article
- Vertebrate GAF/ThPOK: emerging functions in chromatin architecture and transcriptional regulation.Cellular and molecular life sciences : CMLS · 2018Review
- Evolutionary Footprints of Short Tandem Repeats in Avian Promoters.Scientific reports · 2016Article
- Article
- Microsatellite tandem repeats are abundant in human promoters and are associated with regulatory elements.PloS one · 2013Article
- Characterization of simple sequence repeats (SSRs) from Phlebotomus papatasi (Diptera: Psychodidae) expressed sequence tags (ESTs).Parasites & vectors · 2011Article
- A transcribed pseudogene of MYLK promotes cell proliferation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- The yin and yang of repair mechanisms in DNA structure-induced genetic instability.Mutation researchReview
- Review
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Naturally occurring repeat sequences capable of adopting H-DNA structures are abundant in promoters of disease-related genes. In support of this, we found (CT)(22) . (AG)(22) repeats in the promoter of smooth muscle myosin light chain kinase (smMLCK), a key regulator of vascular smooth muscle function. We also found an insertion mutation that adds another six pairs of CT . AG repeats and increases smMLCK promoter activity in spontaneously hypertensive rats (SHR). Therefore, we used the smMLCK promoters from normotensive and hypertensive rats as a model system to determine how CT . AG repeats form H-DNA, an intramolecular triplex, and regulate promoter activity. High-resolution mapping with a chemical probe selective for H-DNA showed that the CT . AG repeats adopt H-DNA structures at a neutral pH. Importantly, the SHR promoter forms longer H-DNA structures than the promoter from normotensive rats. Reconstituting nucleosomes on the promoters, in vitro, showed no difference in nucleosome positioning between the two promoters. However, chromatin immunoprecipitation analyses revealed that histone acetylations are greater in the hypertensive promoter. Thus, our findings suggest that the extended CT . AG repeats in the SHR promoter increase H-DNA structures, histone modifications, and promoter activity of the smMLCK, perhaps contributing to vascular disorders in hypertension.
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