ArticleThe American journal of pathology2007
Responses of nontransformed human hepatocytes to conditional expression of full-length hepatitis C virus open reading frame.
Article in The American journal of pathology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Effects of oxidative stress on viral infections: an overview.Npj viruses · 2025Review
- Iron chelation as a new therapeutic approach to prevent senescence and liver fibrosis progression.Cell death & disease · 2024Article
- Studying the Changes in Physical Functioning and Oxidative Stress-Related Molecules in People Living with HIV after Switching from Triple to Dual Therapy.Antioxidants (Basel, Switzerland) · 2024Article
- Signaling Induced by Chronic Viral Hepatitis: Dependence and Consequences.International journal of molecular sciences · 2022Review
- Oxidative stress, a trigger of hepatitis C and B virus-induced liver carcinogenesis.Oncotarget · 2017Review
- HCV and Oxidative Stress: Implications for HCV Life Cycle and HCV-Associated Pathogenesis.Oxidative medicine and cellular longevity · 2016Review
- Article
- HCV and oxidative stress in the liver.Viruses · 2013Review
- Sorafenib sensitizes hepatocellular carcinoma cells to physiological apoptotic stimuli.Journal of cellular physiology · 2012Article
- Hepatitis C virus proteins modulate microRNA expression and chemosensitivity in malignant hepatocytes.Clinical cancer research : an official journal of the American Association for Cancer Research · 2010Article
- Roles for endocytic trafficking and phosphatidylinositol 4-kinase III alpha in hepatitis C virus replication.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The TRAIL to viral pathogenesis: the good, the bad and the ugly.Current molecular medicine · 2009Review
- HCV induces oxidative and ER stress, and sensitizes infected cells to apoptosis in SCID/Alb-uPA mice.PLoS pathogens · 2009Article
- Article
- Genomic analysis reveals a potential role for cell cycle perturbation in HCV-mediated apoptosis of cultured hepatocytes.PLoS pathogens · 2009Article
- Hepatitis C virus NS5A protein down-regulates the expression of spindle gene Aspm through PKR-p38 signaling pathway.The Journal of biological chemistry · 2008Article
- Review
- Virogenomics: the virus-host interaction revisited.Current opinion in microbiology · 2008Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Hepatitis C virus (HCV) is a major cause of chronic hepatitis that can lead to cirrhosis and hepatocellular carcinoma. To study the effects of HCV protein expression on host cells, we established conditional expression of the full-length open reading frame (ORF) of an infectious cDNA clone of HCV (genotype 1a, H77 strain) in the nontransformed human hepatocyte line cell HH4 using the ecdysone receptor regulatory system. Treatment with the ecdysone analog ponasterone-A induced tightly regulated and dose-dependent full-length HCV ORF expression and properly processed HCV proteins. HCV Core, NS3, and NS5A colocalized in perinuclear regions and associated with the early endosomal protein EEA1. HCV ORF expression caused marked growth inhibition, increased intracellular reactive oxygen species, up-regulation of glutamate-l-cysteine ligase activity, increased glutathione level, and activation of nuclear factor kappaB. Although it was not directly cytotoxic, HCV ORF expression sensitized HH4 cells to Fas at certain concentrations but not to tumor necrosis factor-related apoptosis-inducing ligand. HCV ORF expression in HH4 cells up-regulated genes involved in innate immune response/inflammation and oxidative stress responses and down-regulated cell growth-related genes. Expression of HCV ORF in host cells may contribute to HCV pathogenesis by producing oxidative stress and increasing the expression of genes related to the innate immune response and inflammation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.