ArticleGene therapy2008
The cHS4 chromatin insulator reduces gammaretroviral vector silencing by epigenetic modifications of integrated provirus.
Article in Gene therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- SHIELD: a platform for high-throughput screening of barrier-type DNA elements in human cells.Nature communications · 2023Article
- Epigenetic silencing directs expression heterogeneity of stably integrated multi-transcript unit genetic circuits.Scientific reports · 2021Article
- Review
- Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE).BMC biotechnology · 2019Article
- Spontaneous reactivation of latent HIV-1 promoters is linked to the cell cycle as revealed by a genetic-insulators-containing dual-fluorescence HIV-1-based vector.Scientific reports · 2018Article
- Evaluation of long-term transgene expression in piggyBac-modified human T lymphocytes.Journal of immunotherapy (Hagerstown, Md. : 1997) · 2013Article
- Adjusting the attB site in donor plasmid improves the efficiency of ΦC31 integrase system.DNA and cell biology · 2012Article
- Recent advances in gene therapy for thalassemia.Journal of pharmacy & bioallied sciences · 2012Article
- Prototypic chromatin insulator cHS4 protects retroviral transgene from silencing in Schistosoma mansoni.Transgenic research · 2012Article
- The impact of cHS4 insulators on DNA transposon vector mobilization and silencing in retinal pigment epithelium cells.PloS one · 2012Article
- RNA interference is responsible for reduction of transgene expression after Sleeping Beauty transposase mediated somatic integration.PloS one · 2012Article
- The use of chromatin insulators to improve the expression and safety of integrating gene transfer vectors.Human gene therapy · 2011Review
- Genotoxicity of retroviral hematopoietic stem cell gene therapy.Expert opinion on biological therapy · 2011Review
- Minicircle DNA-based gene therapy coupled with immune modulation permits long-term expression of α-L-iduronidase in mice with mucopolysaccharidosis type I.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Controlled extracellular matrix degradation in breast cancer tumors improves therapy by trastuzumab.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Immuno-therapy with anti-CTLA4 antibodies in tolerized and non-tolerized mouse tumor models.PloS one · 2011Article
- Erythroid-specific expression of β-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells.PloS one · 2011Article
- A functional screen for regulatory elements that improve retroviral vector gene expression.Blood cells, molecules & diseases · 2010Article
- Assessing the risk of T-cell malignancies in mouse models of SCID-X1.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- VEZF1 elements mediate protection from DNA methylation.PLoS genetics · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cHS4 chromatin insulator has been shown to improve the expression of integrating gene transfer vectors by reducing the impact of silencing chromosomal position effects. To better understand the underlying mechanisms of this protection, we investigated the influence of this element on the epigenetic modifications of a gammaretroviral reporter vector. In HT1080 cells, we found that a fourfold increase in the level of green fluorescent protein (GFP) reporter expression from the cHS4-insulated vector was correlated with a twofold increase in acetylation at lysines 9 and 14 of histone H3, but not with CpG methylation. In a mouse bone marrow transduction and transplantation model, we found that a 10-fold increase in the likelihood of GFP expression from the cHS4-insulated vector was correlated with an eightfold increase in histone H3 acetylation, as well as a fourfold decrease in CpG methylation. Histone hyperacetylation peaked at the cHS4 core, and in vivo diminished nearly threefold through the central portion of the vector. Taken together, these studies demonstrate that the cHS4 chromatin insulator reduces gammaretroviral vector silencing by modulating epigenetic modifications of integrated provirus, and identify a specific topological distribution of these modifications that may prove informative for future vector designs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.