ArticleProceedings of the National Academy of Sciences of the United States of America2007
Mutational activation of niche-specific genes provides insight into regulatory networks and bacterial function in a complex environment.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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42 citing papers in PubMed, 1 synthesis or guideline pooled it, 90 citations in OpenAlex.
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- Extending evolutionary forecasts across bacterial species.Proceedings. Biological sciences · 2024Article
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- The Diguanylate Cyclase YfiN of Pseudomonas aeruginosa Regulates Biofilm Maintenance in Response to Peroxide.Journal of bacteriology · 2022Article
- Influence of genomic structural variations and nutritional conditions on the emergence of quorum sensing-dependent gene regulation defects inFrontiers in microbiology · 2022Article
- Transcriptomic analysis ofMicrobial genomics · 2022Article
- The RhizobacteriumMicroorganisms · 2021Article
- Transposon Mutagenesis ofMicroorganisms · 2021Article
- Two Homologues of the Global Regulator Csr/Rsm Redundantly Control Phaseolotoxin Biosynthesis and Virulence in the Plant PathogenMicroorganisms · 2020Article
- Bifidobacterial biofilm formation is a multifactorial adaptive phenomenon in response to bile exposure.Scientific reports · 2020Article
- Elevated exopolysaccharide levels in Pseudomonas aeruginosa flagellar mutants have implications for biofilm growth and chronic infections.PLoS genetics · 2020Article
- Genetic and molecular characterization of multicomponent resistance ofLife science alliance · 2020Article
- Causes and Biophysical Consequences of Cellulose Production by Pseudomonas fluorescens SBW25 at the Air-Liquid Interface.Journal of bacteriology · 2019Article
- Article
- NfiR, a New Regulatory Noncoding RNA (ncRNA), Is Required in Concert with the NfiS ncRNA for Optimal Expression of Nitrogenase Genes in Pseudomonas stutzeri A1501.Applied and environmental microbiology · 2019Article
- Repeated Phenotypic Evolution by Different Genetic Routes in Pseudomonas fluorescens SBW25.Molecular biology and evolution · 2019Article
- AmrZ and FleQ Co-regulate Cellulose Production inFrontiers in microbiology · 2019Article
- Evolutionary convergence in experimental Pseudomonas populations.The ISME journal · 2017Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of the plant-colonizing bacterium Pseudomonas fluorescens SBW25 harbors a subset of genes that are expressed specifically on plant surfaces. The function of these genes is central to the ecological success of SBW25, but their study poses significant challenges because no phenotype is discernable in vitro. Here, we describe a genetic strategy with general utility that combines suppressor analysis with IVET (SPyVET) and provides a means of identifying regulators of niche-specific genes. Central to this strategy are strains carrying operon fusions between plant environment-induced loci (EIL) and promoterless 'dapB. These strains are prototrophic in the plant environment but auxotrophic on laboratory minimal medium. Regulatory elements were identified by transposon mutagenesis and selection for prototrophs on minimal medium. Approximately 10(6) mutants were screened for each of 27 strains carrying 'dapB fusions to plant EIL and the insertion point for the transposon determined in approximately 2,000 putative regulator mutants. Regulators were functionally characterized and used to provide insight into EIL phenotypes. For one strain carrying a fusion to the cellulose-encoding wss operon, five different regulators were identified including a diguanylate cyclase, the flagella activator, FleQ, and alginate activator, AmrZ (AlgZ). Further rounds of suppressor analysis, possible by virtue of the SPyVET strategy, revealed an additional two regulators including the activator AlgR, and allowed the regulatory connections to be determined.
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