ArticleJournal of virology2008
Protease activity, self interaction, and small interfering RNA binding of the silencing suppressor p1b from cucumber vein yellowing ipomovirus.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Virus meets RNAi. Symposium on antiviral applications of RNA interference.EMBO reports · 2008Trial
- Discovery of novel viruses related to theVirus evolution · 2026Article
- Proteome expansion in the Potyviridae evolutionary radiation.FEMS microbiology reviews · 2022Review
- An Importin-β-like Protein fromViruses · 2021Article
- P1 ofJournal of virology · 2021Article
- Functional analysis of apple stem pitting virus coat protein variants.Virology journal · 2019Article
- Cassava brown streak disease: historical timeline, current knowledge and future prospects.Molecular plant pathology · 2018Review
- Plant Viral Proteases: Beyond the Role of Peptide Cutters.Frontiers in plant science · 2018Review
- The P1N-PISPO trans-Frame Gene of Sweet Potato Feathery Mottle Potyvirus Is Produced during Virus Infection and Functions as an RNA Silencing Suppressor.Journal of virology · 2016Article
- Key importance of small RNA binding for the activity of a glycine-tryptophan (GW) motif-containing viral suppressor of RNA silencing.The Journal of biological chemistry · 2015Article
- Novel Drosophila viruses encode host-specific suppressors of RNAi.PLoS pathogens · 2014Article
- Identification of the amino acid residues and domains in the cysteine-rich protein of Chinese wheat mosaic virus that are important for RNA silencing suppression and subcellular localization.Molecular plant pathology · 2013Article
- Article
- Eggplant mild leaf mottle virus (EMLMV), a new putative member of the genus Ipomovirus that harbors an HC-Pro gene.Virus genes · 2012Article
- The VP3 factor from viruses of Birnaviridae family suppresses RNA silencing by binding both long and small RNA duplexes.PloS one · 2012Article
- The specific binding to 21-nt double-stranded RNAs is crucial for the anti-silencing activity of Cucumber vein yellowing virus P1b and perturbs endogenous small RNA populations.RNA (New York, N.Y.) · 2011Article
- P1 peptidase--a mysterious protein of family Potyviridae.Journal of biosciences · 2011Review
- Viral protein inhibits RISC activity by argonaute binding through conserved WG/GW motifs.PLoS pathogens · 2010Article
- Constraints to virus infection in Nicotiana benthamiana plants transformed with a potyvirus amplicon.BMC plant biology · 2010Article
- Bymovirus reverse genetics: requirements for RNA2-encoded proteins in systemic infection.Molecular plant pathology · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RNA silencing pathway mediated by small interfering RNAs (siRNAs) plays an important antiviral role in eukaryotes. To counteract this defense barrier, a large number of plant viruses express proteins with RNA silencing suppression activity. Recently, it was reported that the ipomovirus Cucumber vein yellowing virus (CVYV), which lacks the typical silencing suppressor of members of the family Potyviridae, i.e., HCPro, has a duplicated P1 coding sequence and that the downstream P1 copy, named P1b, has silencing suppression activity. In this study, we provide experimental evidence that P1b is a serine protease that self-cleaves at its C terminus but that its proteolytic activity is not essential for silencing suppression. In contrast, a putative zinc finger and a conserved basic motif in the N-terminal region of the protein are required for efficient silencing suppression. In vitro gel filtration-fast protein liquid chromatography and in vivo bimolecular fluorescence complementation assays showed that P1b binds itself to form oligomeric structures and that the zinc finger-like motif is essential for the self interaction. Moreover, we observed that CVYV P1b forms complexes with synthetic siRNAs, and this ability correlated with both silencing suppression activity and enhancement of Potato virus X pathogenicity in a mutational analysis. Together, these results suggest that CVYV P1b resembles potyviral HCPro and other viral proteins in interfering RNA silencing by preventing siRNA loading into the RNA-induced silencing complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.