ArticleMolecular biotechnology2008
Interactive fluorophore and quencher pairs for labeling fluorescent nucleic acid hybridization probes.
Article in Molecular biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Locked Nucleic Acid and Minor Groove Binder Probes Improve the Specificity of Isothermal Amplification Detection.Analytical chemistry · 2026Article
- Universal primer mediated multiplex asymmetric PCR coupled with hybridization-based electrochemical biosensing for rapid, simultaneous and label-free detection of multiple pathogens.Mikrochimica acta · 2026Article
- Spectral fingerprint diagnosis: Spatially independent analysis of biomarker patterns in homogeneous systems.Science advances · 2025Article
- Cas12a Cis-cleavage mediated lateral flow assay enables multiplex and ultra-specific nucleic acid detection.Nature communications · 2025Article
- Fluorescence origami paper-based analytical device based on strand displacement assay for SARS-CoV-2 cDNA detection.Mikrochimica acta · 2025Article
- Fluorogenic oligonucleotide cleavage probes with a branched linker improve RNA detection.Nucleic acids research · 2025Article
- Magnetoimpedance Biosensors and Real-Time Healthcare Monitors: Progress, Opportunities, and Challenges.Biosensors · 2022Review
- Highly multiplex PCR assays by coupling the 5'-flap endonuclease activity ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Rapid and Easy-to-Perform Method of Nucleic-Acid Based Dengue Virus Diagnosis Using Fluorescence-Based Molecular Beacons.Biosensors · 2021Article
- Design and Synthesis of Cathepsin-K-Activated Osteoadsorptive Fluorogenic Sentinel (OFS) Probes for Detecting Early Osteoclastic Bone Resorption in a Multiple Myeloma Mouse Model.Bioconjugate chemistry · 2021Article
- Super-beacons: Open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy.Traffic (Copenhagen, Denmark) · 2020Article
- TWJ-Screen: an isothermal screening assay to assess ligand/DNA junction interactions in vitro.Nucleic acids research · 2018Article
- Fluorogenic PNA probes.Beilstein journal of organic chemistry · 2018Review
- A novel multiplex isothermal amplification method for rapid detection and identification of viruses.Scientific reports · 2015Article
- Evaluation of impermeant, DNA-binding dye fluorescence as a real-time readout of eukaryotic cell toxicity in a high throughput screening format.Assay and drug development technologies · 2014Article
- Synthesis and optical properties of pyrrolidinyl peptide nucleic acid carrying a clicked Nile red label.Beilstein journal of organic chemistry · 2014Article
- Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency.Mikrochimica acta · 2014Article
- Allosterically tunable, DNA-based switches triggered by heavy metals.Journal of the American Chemical Society · 2013Article
- Ligand-induced changes in T box antiterminator RNA stability.Chemical biology & drug design · 2012Article
- Molecular beacons in diagnostics.F1000 medicine reports · 2012Article
Corrections and comments
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1 author.
Funding
Abstract
The use of fluorescent nucleic acid hybridization probes that generate a fluorescence signal only when they bind to their target enables real-time monitoring of nucleic acid amplification assays. Real-time nucleic acid amplification assays markedly improves the ability to obtain qualitative and quantitative results. Furthermore, these assays can be carried out in sealed tubes, eliminating carryover contamination. Fluorescent nucleic acid hybridization probes are available in a wide range of different fluorophore and quencher pairs. Multiple hybridization probes, each designed for the detection of a different nucleic acid sequence and each labeled with a differently colored fluorophore, can be added to the same nucleic acid amplification reaction, enabling the development of high-throughput multiplex assays. In order to develop robust, highly sensitive and specific real-time nucleic acid amplification assays it is important to carefully select the fluorophore and quencher labels of hybridization probes. Selection criteria are based on the type of hybridization probe used in the assay, the number of targets to be detected, and the type of apparatus available to perform the assay. This article provides an overview of different aspects of choosing appropriate labels for the different types of fluorescent hybridization probes used with different types of spectrofluorometric thermal cyclers currently available.
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