ArticleNature methods2007
In situ proteolysis for protein crystallization and structure determination.
Article in Nature methods, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers.
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Who cites it
136 citing papers in PubMed.
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- Single-Domain Antibodies as Antibody-Drug Conjugates: From Promise to Practice-A Systematic Review.Cancers · 2024Review
- The NTE domain of PTENα/β promotes cancer progression by interacting with WDR5 via its SSSRRSS motif.Cell death & disease · 2024Article
- Structural and functional validation of a highly specific Smurf2 inhibitor.Protein science : a publication of the Protein Society · 2024Article
- Polymorphic nanobody crystals as long-acting intravitreal therapy for wet age-related macular degeneration.Bioengineering & translational medicine · 2023Article
- Rational engineering enables co-crystallization and structural determination of the HIV-1 matrix-tRNA complex.STAR protocols · 2022Article
- Structure and dsRNA-binding activity of the Birnavirus Drosophila X Virus VP3 protein.Journal of virology · 2021Article
- Comprehensive analysis of IncC plasmid conjugation identifies a crucial role for the transcriptional regulator AcaB.Nature microbiology · 2020Article
- Envelope stress responses defend against type six secretion system attacks independently of immunity proteins.Nature microbiology · 2020Article
- A simple and rapid pipeline for identification of receptor-binding sites on the surface proteins of pathogens.Scientific reports · 2020Article
- In Situ Proteolysis Condition-Induced Crystallization of the XcpVWX Complex in Different Lattices.International journal of molecular sciences · 2020Article
- Structural analysis of biological targets by host:guest crystal lattice engineering.Scientific reports · 2019Article
- Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.Nature communications · 2019Article
- A threonyl-tRNA synthetase-mediated translation initiation machinery.Nature communications · 2019Article
- Uncoiling CNLs: Structure/Function Approaches to Understanding CC Domain Function in Plant NLRs.Plant & cell physiology · 2018Review
- Critical evaluation of bioinformatics tools for the prediction of protein crystallization propensity.Briefings in bioinformatics · 2018Review
- T4 lysozyme-facilitated crystallization of the human molybdenum cofactor-dependent enzyme mARC.Acta crystallographica. Section F, Structural biology communications · 2018Article
- In situ proteolysis of an N-terminal His tag with thrombin improves the diffraction quality of human aldo-keto reductase 1C3 crystals.Acta crystallographica. Section F, Structural biology communications · 2018Article
76 more citing papers are in PubMed but not listed here.
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Authors and funding
71 authors.
Funding
Abstract
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
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