ArticleThe Plant cell2007
Profilin is essential for tip growth in the moss Physcomitrella patens.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 141 citations in OpenAlex.
- TON1 and FASS are required to recruit myosin VIII, a cell division guidance factor.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Essential developmental processes in Physcomitrium patens require distinct levels of total activity provided by functionally redundant PpROP GTPases.The New phytologist · 2025Article
- Complex sphingolipid metabolism impacts cell division and plasmodesmal development in the moss Physcomitrium patens.Plant physiology · 2025Article
- Novel small molecules disrupting polarized cell expansion and development in the moss,Plant biotechnology (Tokyo, Japan) · 2025Article
- Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels.Nature communications · 2025Article
- Dynamic Membrane Lipid Changes inBiology · 2024Article
- Cellulose synthase-like D movement in the plasma membrane requires enzymatic activity.The Journal of cell biology · 2023Article
- Armadillo repeat-containing kinesin represents the versatile plus-end-directed transporter in Physcomitrella.Nature plants · 2023Article
- Control of Plant Cell Growth and Proliferation by MO25A, a Conserved Major Component of the Mammalian Sterile 20-Like Kinase Pathway.Plant & cell physiology · 2023Article
- Cellulose synthesis complexes are homo-oligomeric and hetero-oligomeric in Physcomitrium patens.Plant physiology · 2022Article
- COPII Sec23 proteins form isoform-specific endoplasmic reticulum exit sites with differential effects on polarized growth.The Plant cell · 2022Article
- The bryophytes Physcomitrium patens and Marchantia polymorpha as model systems for studying evolutionary cell and developmental biology in plants.The Plant cell · 2022Review
- Physcomitrium patens Protoplasting and Protoplast Transfection.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Myosin XI drives polarized growth by vesicle focusing and local enrichment of F-actin in Physcomitrium patens.Plant physiology · 2021Article
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- Article
- Rab-E and its interaction with myosin XI are essential for polarised cell growth.The New phytologist · 2021Article
- A Fully Functional ROP Fluorescent Fusion Protein Reveals Roles for This GTPase in Subcellular and Tissue-Level Patterning.The Plant cell · 2020Article
- Robust Survival-Based RNA Interference of Gene Families Using in Tandem Silencing of Adenine Phosphoribosyltransferase.Plant physiology · 2020Article
- The MossThe Plant cell · 2020Review
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The actin cytoskeleton is critical for tip growth in plants. Profilin is the main monomer actin binding protein in plant cells. The moss Physcomitrella patens has three profilin genes, which are monophyletic, suggesting a single ancestor for plant profilins. Here, we used RNA interference (RNAi) to determine the loss-of-function phenotype of profilin. Reduction of profilin leads to a complete loss of tip growth and a partial inhibition of cell division, resulting in plants with small rounded cells and fewer cells. We silenced all profilins by targeting their 3' untranslated region sequences, enabling complementation analyses by expression of profilin coding sequences. We show that any moss or a lily (Lilium longiflorum) profilin support tip growth. Profilin with a mutation in its actin binding site is unable to rescue profilin RNAi, while a mutation in the poly-l-proline binding site weakly rescues. We show that moss tip growing cells contain a prominent subapical cortical F-actin structure composed of parallel actin cables. Cells lacking profilin lose this structure; instead, their F-actin is disorganized and forms polarized cortical patches. Plants expressing the actin and poly-l-proline binding mutants exhibited similar F-actin disorganization. These results demonstrate that profilin and its binding to actin are essential for tip growth. Additionally, profilin is not needed for formation of F-actin, but profilin and its interactions with actin and poly-l-proline ligands are required to properly organize F-actin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.