ArticlePlant physiology2007
A set of modular binary vectors for transformation of cereals.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
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97 citing papers in PubMed, 233 citations in OpenAlex.
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- Virulence on Pm4 kinase-based resistance is determined by two divergent wheat powdery mildew effectors.Nature plants · 2026Article
- Effects of suppression of phosphate transporter 4;4 on COJournal of plant research · 2025Article
- BdNRT2A and BdNRT3.2 Are the Major Components of the High-Affinity Nitrate Transport System inPlant direct · 2025Article
- Population genomics and molecular epidemiology of wheat powdery mildew in Europe.PLoS biology · 2025Article
- The Wheat NLR Protein PM3b Localises to Endoplasmic Reticulum-Plasma Membrane Contact Sites and Interacts With AVRPM3Molecular plant pathology · 2025Article
- Avirulence depletion assay: Combining R gene-mediated selection with bulk sequencing for rapid avirulence gene identification in wheat powdery mildew.PLoS pathogens · 2025Article
- Functional Characterization of the CiliateAntioxidants (Basel, Switzerland) · 2024Article
- Over-accumulation of chloroplast-nucleus located WHIRLY1 in barley leads to a decrease in growth and an enhanced stress resistance.The Plant journal : for cell and molecular biology · 2024Article
- Melatonin-Regulated Chaperone Binding Protein Plays a Key Role in Cadmium Stress Tolerance in Rice, Revealed by the Functional Characterization of a Novel SerotoninInternational journal of molecular sciences · 2024Article
- Genome-wide association analysis uncovers rice blast resistance alleles of Ptr and Pia.Communications biology · 2024Article
- Wheat zinc finger protein TaZF interacts with both the powdery mildew AvrPm2 protein and the corresponding wheat Pm2a immune receptor.Plant communications · 2024Article
- The SpRY Cas9 variant release the PAM sequence constraint for genome editing in the model plant Physcomitrium patens.Transgenic research · 2024Article
- A dominant-negative avirulence effector of the barley powdery mildew fungus provides mechanistic insight into barley MLA immune receptor activation.Journal of experimental botany · 2023Article
- Structural polymorphisms within a common powdery mildew effector scaffold as a driver of coevolution with cereal immune receptors.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Ectopic overexpression of TaHsfA5 promotes thermomorphogenesis in Arabidopsis thaliana and thermotolerance in Oryza sativa.Plant molecular biology · 2023Article
- Clathrin-mediated endocytosis facilitates the internalization of Magnaporthe oryzae effectors into rice cells.The Plant cell · 2023Article
37 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic transformation of crop plants offers the possibility of testing hypotheses about the function of individual genes as well as the exploitation of transgenes for targeted trait improvement. However, in most cereals, this option has long been compromised by tedious and low-efficiency transformation protocols, as well as by the lack of versatile vector systems. After having adopted and further improved the protocols for Agrobacterium-mediated stable transformation of barley (Hordeum vulgare) and wheat (Triticum aestivum), we now present a versatile set of binary vectors for transgene overexpression, as well as for gene silencing by double-stranded RNA interference. The vector set is offered with a series of functionally validated promoters and allows for rapid integration of the desired genes or gene fragments by GATEWAY-based recombination. Additional in-built flexibility lies in the choice of plant selectable markers, cassette orientation, and simple integration of further promoters to drive specific expression of genes of interest. Functionality of the cereal vector set has been demonstrated by transient as well as stable transformation experiments for transgene overexpression, as well as for targeted gene silencing in barley.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.