ArticleApplied and environmental microbiology2008
Analysis of fungal flora in indoor dust by ribosomal DNA sequence analysis, quantitative PCR, and culture.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed.
- Characterization of Airborne Fungal Diversity in Nunavik Housing Using Activated Air Sampling.ACS ES&T air · 2026Article
- Decoding the genome of the basidiomycetous yeast Vishniacozyma victoriae D19: a promising fungal model for biotechnology.BMC genomics · 2026Article
- The saprotrophic dimension ofIMA fungus · 2026Article
- Dynamic airborne mycobiome in the metropolitan city transit system is driven by seasonality and station type.Microbiology spectrum · 2025Article
- Associations Between Indoor Fungal Community Structures and Environmental Factors: Insights from the Evidence-Driven Indoor Air-Quality Improvement Study.Journal of fungi (Basel, Switzerland) · 2025Article
- The Human Mycobiome.Current topics in microbiology and immunology · 2025Review
- Examination of Aeroallergen Sensitization Patterns in Southeastern Louisiana.Ochsner journal · 2025Article
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- Two newFrontiers in microbiology · 2024Article
- Seasonal Variation in Fungi in Beach Sand in Summertime: Stintino (Italy).International journal of environmental research and public health · 2023Article
- PersistingFrontiers in cellular and infection microbiology · 2023Article
- Da Vinci's yeast: Blastobotrys davincii f.a., sp. nov.Yeast (Chichester, England) · 2023Article
- Microbial Contamination of Bedding Material: One Health in Poultry Production.International journal of environmental research and public health · 2022Review
- The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates.Microorganisms · 2022Article
- Vishniacozyma victoriae (syn. Cryptococcus victoriae) in the homes of asthmatic and non-asthmatic children in New York City.Journal of exposure science & environmental epidemiology · 2022Article
- Spatiotemporal variation of the indoor mycobiome in daycare centers.Microbiome · 2021Article
- Comparison of DNA sequencing and morphological identification techniques to characterize environmental fungal communities.Scientific reports · 2021Article
- Dust accumulated fungi in air-conditioning system: Findings based on field and laboratory experiments.Building simulation · 2021Article
- Biological effects of inhaled hydraulic fracturing sand dust. III. Cytotoxicity and pro-inflammatory responses in cultured murine macrophage cells.Toxicology and applied pharmacology · 2020Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years increasing attention has been given to the potential health effects of fungal exposure in indoor environments. We used large-scale sequencing of the fungal internal transcribed spacer region (ITS) of nuclear ribosomal DNA to describe the mycoflora of two office buildings over the four seasons. DNA sequencing was complemented by cultivation, ergosterol determination, and quantitative PCR analyses. Sequences of 1,339 clones were clustered into 394 nonredundant fungal operational taxonomical units containing sequences from 18 fungal subclasses. The observed flora differed markedly from that recovered by cultivation, the major differences being the near absence of several typical indoor mold genera such as Penicillium and Aspergillus spp. and a high prevalence of basidiomycetes in clone libraries. A total of 55% of the total diversity constituted of unidentifiable ITS sequences, some of which may represent novel fungal species. Dominant species were Cladosporium cladosporioides and C. herbarum, Cryptococcus victoriae, Leptosphaerulina americana and L. chartarum, Aureobasidium pullulans, Thekopsora areolata, Phaeococcomyces nigricans, Macrophoma sp., and several Malassezia species. Seasonal differences were observed for community composition, with ascomycetous molds and basidiomycetous yeasts predominating in the winter and spring and Agaricomycetidae basidiomycetes predominating in the fall. The comparison of methods suggested that the cloning, cultivation, and quantitative PCR methods complemented each other, generating a more comprehensive picture of fungal flora than any of the methods would give alone. The current restrictions of the methods are discussed.
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