ArticleBiophysical journal2008
Cholesterol level regulates endosome motility via Rab proteins.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 75 citations in OpenAlex.
- Lipid Composition Drives Mutant Huntingtin Dimerization and Membrane Association: Insights from Computational Simulations.Molecules (Basel, Switzerland) · 2026Article
- Characterization of the Effect of α-Lipoic Acid in Human Macrophages Infected withInternational journal of molecular sciences · 2026Article
- Cholesterol-dependent control of endosomal escape regulates intracellular trafficking of small interfering RNA therapeutics and interactions with small molecule drugs.The Journal of pharmacology and experimental therapeutics · 2026Article
- Cell cycle-regulated tug-of-war between microtubule motors positions major trafficking organelles.bioRxiv : the preprint server for biology · 2025Article
- Lysosomal Repair in Health and Disease.Journal of cellular physiology · 2025Review
- Fluorogenic RNA-based biomaterials for imaging and tracking the cargo of extracellular vesicles.Journal of controlled release : official journal of the Controlled Release Society · 2024Article
- Alterations in Proteostasis Mechanisms in Niemann-Pick Type C Disease.International journal of molecular sciences · 2024Review
- Modulating cholesterol-rich lipid rafts to disrupt influenza A virus infection.Frontiers in immunology · 2022Review
- Extracellular Vesicles-The Next Frontier in Endocrinology.Endocrinology · 2021Review
- Function of the endolysosomal network in cholesterol homeostasis and metabolic-associated fatty liver disease (MAFLD).Molecular metabolism · 2021Review
- Endosome motility defects revealed at super-resolution in live cells using HIDE probes.Nature chemical biology · 2020Article
- Apolipoprotein E4 genotype compromises brain exosome production.Brain : a journal of neurology · 2019Article
- Folate action in nervous system development and disease.Developmental neurobiology · 2018Review
- Lipid metabolism fattens up hedgehog signaling.BMC biology · 2017Review
- A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation.Nature cell biology · 2016Article
- Prion infection impairs lysosomal degradation capacity by interfering with rab7 membrane attachment in neuronal cells.Scientific reports · 2016Article
- A Journey of Cytolethal Distending Toxins through Cell Membranes.Frontiers in cellular and infection microbiology · 2016Review
- Increasing membrane cholesterol of neurons in culture recapitulates Alzheimer's disease early phenotypes.Molecular neurodegeneration · 2014Article
- Rab proteins and the compartmentalization of the endosomal system.Cold Spring Harbor perspectives in biology · 2014Review
- Influenza a virus assembly intermediates fuse in the cytoplasm.PLoS pathogens · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of cholesterol in the regulation of endosome motility was investigated by monitoring the intracellular trafficking of endocytosed folate receptors (FRs) labeled with fluorescent folate conjugates. Real-time fluorescence imaging of HeLa cells transfected with green fluorescent protein-tubulin revealed that FR-containing endosomes migrate along microtubules. Moreover, microinjection with antibodies that inhibit microtubule-associated motor proteins demonstrated that dynein and kinesin I participate in the delivery of FR-containing endosomes to the perinuclear area and plasma membrane, respectively. Further, single-particle tracking analysis revealed bidirectional motions of FR endosomes, mediated by dynein and kinesin motors associated with the same endosome. These experimental tools allowed us to use FR-containing endosomes to evaluate the impact of cholesterol on intracellular membrane trafficking. Lowering plasma membrane cholesterol by metabolic depletion or methyl-beta-cyclodextrin extraction was found to both increase FR-containing endosome motility and change endosome distribution from colocalization with Rab7 to colocalization with Rab4. These data provide evidence that cholesterol regulates intracellular membrane trafficking via modulation of the distribution of low molecular weight G-proteins that are adaptors for microtubule motors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.