ArticleBiophysical journal2008
Elastic membrane heterogeneity of living cells revealed by stiff nanoscale membrane domains.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 89 citations in OpenAlex.
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- Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis.Stem cell research & therapy · 2019Article
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- Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure.European biophysics journal : EBJ · 2017Article
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- Cholesterol Regulates Monocyte Rolling through CD44 Distribution.Biophysical journal · 2017Article
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- Cellular cholesterol regulates monocyte deformation.Journal of biomechanics · 2017Article
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- Interaction of membrane/lipid rafts with the cytoskeleton: impact on signaling and function: membrane/lipid rafts, mediators of cytoskeletal arrangement and cell signaling.Biochimica et biophysica acta · 2014Review
- Fluid shear stress induces the clustering of heparan sulfate via mobility of glypican-1 in lipid rafts.American journal of physiology. Heart and circulatory physiology · 2013Article
- Multiparametric imaging of biological systems by force-distance curve-based AFM.Nature methods · 2013Review
- Sphingomyelin organization is required for vesicle biogenesis at the Golgi complex.The EMBO journal · 2012Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many approaches have been developed to characterize the heterogeneity of membranes in living cells. In this study, the elastic properties of specific membrane domains in living cells are characterized by atomic force microscopy. Our data reveal the existence of heterogeneous nanometric scale domains with specific biophysical properties. We focused on glycosylphosphatidylinositol (GPI)-anchored proteins, which play an important role in membrane trafficking and cell signaling under both physiological and pathological conditions and which are known to partition preferentially into cholesterol-rich microdomains. We demonstrate that these GPI-anchored proteins reside within domains that are stiffer than the surrounding membrane. In contrast, membrane domains containing the transferrin receptor, which does not associate with cholesterol-rich regions, manifest no such feature. The heightened stiffness of GPI domains is consistent with existing data relating to the specific condensation of lipids and the slow diffusion rates of lipids and proteins therein. Our quantitative data may forge the way to unveiling the links that exist between membrane stiffness, molecular diffusion, and signaling activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.