ArticleBiophysical journal2008
Toward physiological conditions for cell analyses: forces of heart muscle cells suspended between elastic micropillars.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
- Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications.Small science · 2025Review
- Noninvasive Quantification of Contractile Dynamics in Cardiac Cells, Spheroids, and Organs-on-a-Chip Using High-Frequency Ultrasound.ACS nano · 2024Article
- Continuous contractile force and electrical signal recordings of 3D cardiac tissue utilizing conductive hydrogel pillars on a chip.Materials today. Bio · 2023Article
- Article
- Development of a simple and versatile in vitro method for production, stimulation, and analysis of bioengineered muscle.PloS one · 2022Article
- Soft Polymer-Based Technique for Cellular Force Sensing.Polymers · 2021Review
- Biosensors Based on Mechanical and Electrical Detection Techniques.Sensors (Basel, Switzerland) · 2020Review
- Cell migration on microposts with surface coating and confinement.Bioscience reports · 2019Article
- Genetically Engineered Phage Induced Selective H9c2 Cardiomyocytes Patterning in PDMS Microgrooves.Materials (Basel, Switzerland) · 2017Article
- Mechanotransduction and Metabolism in Cardiomyocyte Microdomains.BioMed research international · 2016Review
- Micro- and nanodevices integrated with biomolecular probes.Biotechnology advances · 2015Review
- Measurement of antibody effects on cellular function of isolated cardiomyocytes.Journal of visualized experiments : JoVE · 2013Article
- Sacrificial layer technique for axial force post assay of immature cardiomyocytes.Biomedical microdevices · 2013Article
- Microdomain heterogeneity in 3D affects the mechanics of neonatal cardiac myocyte contraction.Biomechanics and modeling in mechanobiology · 2013Article
- Contractility assessment in enzymatically isolated cardiomyocytes.Biophysical reviews · 2012Review
- Engineering microscale topographies to control the cell-substrate interface.Biomaterials · 2012Review
- Substrate stiffness increases twitch power of neonatal cardiomyocytes in correlation with changes in myofibril structure and intracellular calcium.Biophysical journal · 2011Article
- Micromechanical regulation in cardiac myocytes and fibroblasts: implications for tissue remodeling.Pflugers Archiv : European journal of physiology · 2011Review
- Mechanobiology of platelets: techniques to study the role of fluid flow and platelet retraction forces at the micro- and nano-scale.International journal of molecular sciences · 2011Review
- Mechanics and contraction dynamics of single platelets and implications for clot stiffening.Nature materials · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Almost each mammalian cell permanently applies forces to its environment. These forces are essential for many vital processes such as tissue formation or cell movement. In turn, the environmental conditions of cells strongly affect force production. Here we report on the development of an array of elastomeric micropillars as cellular environment. Within these micropillar arrays, we cultivated rat heart muscle cells (cardiac myocytes). For lattice constants between 20 and 30 mum, cells strongly preferred spanning between the elastic micropillars over adhering to the underlying flat substrate. In addition, the architectures of the cytoskeleton and of protein complexes formed for adhesion were strongly dependent on the environment of the cell. On flat parts of the substrates, we observed prominent stress fibers and focal adhesion sites. In contrast, cells suspended between micropillars exhibited well organized myofibers and costameric adhesions at the locations of Z-bands. These observations argue for close-to-nature environmental conditions within micropillar arrays. Resting as well as contraction forces of myocytes resulted in measurable pillar bending. Using an approximate theoretical treatment of elastically founded micropillars, we calculated average cell forces of 140 nN in the relaxed and 400 nN in the contracted state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.