ArticleNucleic acids research2008
RNA secondary structure regulates the translation of sxy and competence development in Haemophilus influenzae.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Diverse regulatory pathways modulate bet hedging of competence induction in epigenetically-differentiated phase variants of Streptococcus pneumoniae.Nucleic acids research · 2023Article
- The molecular mechanism for carbon catabolite repression of the chitin response in Vibrio cholerae.PLoS genetics · 2023Article
- Microevolution in response to transient heme-iron restriction enhances intracellular bacterial community development and persistence.PLoS pathogens · 2018Article
- A streptomycin resistance marker inPeerJ · 2018Article
- Direct regulation of the natural competence regulator gene tfoX by cyclic AMP (cAMP) and cAMP receptor protein (CRP) in Vibrios.Scientific reports · 2015Article
- A 5' UTR-mediated translational efficiency mechanism inhibits the Candida albicans morphological transition.Molecular microbiology · 2014Article
- Natural competence and the evolution of DNA uptake specificity.Journal of bacteriology · 2014Review
- Bacterial transformation: distribution, shared mechanisms and divergent control.Nature reviews. Microbiology · 2014Review
- The availability of purine nucleotides regulates natural competence by controlling translation of the competence activator Sxy.Molecular microbiology · 2013Article
- Multiple Pathways of Genome Plasticity Leading to Development of Antibiotic Resistance.Antibiotics (Basel, Switzerland) · 2013Review
- Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons.Journal of bacteriology · 2012Article
- Connecting environment and genome plasticity in the characterization of transformation-induced SOS regulation and carbon catabolite control of the Vibrio cholerae integron integrase.Journal of bacteriology · 2012Article
- Natural DNA uptake by Escherichia coli.PloS one · 2012Article
- Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae.Journal of bacteriology · 2011Article
- Sxy induces a CRP-S regulon in Escherichia coli.Journal of bacteriology · 2009Article
- RNA secondary structure regulates the translation of sxy and competence development in Haemophilus influenzae.Nucleic acids research · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sxy (tfoX) gene product is the central regulator of DNA uptake by naturally competent gamma-proteobacteria such as Haemophilus influenzae, Vibrio cholerae and probably Escherichia coli. However, the mechanisms regulating sxy gene expression are not understood despite being key to understanding the physiological role of DNA uptake. We have isolated mutations in H. influenzae sxy that greatly elevate translation and thus cause competence to develop in otherwise non-inducing conditions (hypercompetence). In vitro nuclease analysis confirmed the existence of an extensive secondary structure at the 5' end of sxy mRNA that sequesters the ribosome-binding site and start codon in a stem-loop. All of the hypercompetence mutations reduced mRNA base pairing, and one was shown to cause a global destabilization that increased translational efficiency. Conversely, mutations engineered to add mRNA base pairs strengthened the secondary structure, resulting in reduced translational efficiency and greatly reduced competence for genetic transformation. Transfer of wild-type cells to starvation medium improved translational efficiency of sxy while independently triggering the sugar starvation regulator (CRP) to stimulate transcription at the sxy promoter. Thus, mRNA secondary structure is responsive to conditions where DNA uptake will be favorable, and transcription of sxy is simultaneously enhanced if CRP activation signals that energy supplies are limited.
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