ArticleDNA repair2008
Removal of deoxyinosine from the Escherichia coli chromosome as studied by oligonucleotide transformation.
Article in DNA repair, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- DREAMS illuminates spatial DNA and RNA modification landscapes.Nature cell biology · 2026Article
- Biological role of the major AP (abasic site) endonuclease of an archaeon from geothermal environments.Extremophiles : life under extreme conditions · 2022Article
- Genetic Control of Oxidative Mutagenesis inJournal of bacteriology · 2020Article
- Evolution of Inosine-Specific Endonuclease V from Bacterial DNase to Eukaryotic RNase.Molecular cell · 2019Article
- Breaking the speed limit with multimode fast scanning of DNA by Endonuclease V.Nature communications · 2018Article
- Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis.Journal of visualized experiments : JoVE · 2018Article
- Detection of Reaction Intermediates in MgMethods in enzymology · 2017Article
- Article
- Crystal structure of E. coli endonuclease V, an essential enzyme for deamination repair.Scientific reports · 2015Article
- Deoxyinosine repair in nuclear extracts of human cells.Cell & bioscience · 2015Article
- Endonuclease V: an unusual enzyme for repair of DNA deamination.Cellular and molecular life sciences : CMLS · 2013Review
- Mispaired rNMPs in DNA are mutagenic and are targets of mismatch repair and RNases H.Nature structural & molecular biology · 2011Article
- Kinetic mechanism for the excision of hypoxanthine by Escherichia coli AlkA and evidence for binding to DNA ends.Biochemistry · 2011Article
- Dissecting endonuclease and exonuclease activities in endonuclease V from Thermotoga maritima.Nucleic acids research · 2011Article
- NUDT16 is a (deoxy)inosine diphosphatase, and its deficiency induces accumulation of single-strand breaks in nuclear DNA and growth arrest.Nucleic acids research · 2010Article
- Structures of endonuclease V with DNA reveal initiation of deaminated adenine repair.Nature structural & molecular biology · 2009Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Deoxyinosine (dI) is produced in DNA by the hydrolytic or nitrosative deamination of deoxyadenosine. It is excised in a repair pathway that is initiated by endonuclease V, the product of the nfi gene. The repair was studied in vivo using high-efficiency oligonucleotide transformation mediated by the Beta protein of bacteriophage lambda in a mismatch repair-deficient host. Escherichia coli was transformed with oligonucleotides containing a selectable A-G base substitution mutation. When the mutagenic dG was replaced by a dI in the oligonucleotide, it lost 93-99% of its transforming ability in an nfi(+) cell, but it remained fully functional in an nfi mutant. Therefore, endonuclease V is responsible for most of the removal of deoxyinosine from DNA. New nfi mutants were isolated based on the strong selection provided by their tolerance for transformation by dI-containing DNA. The repair patch for dI was then measured by determining how close to the transforming dG residue a dI could be placed in the oligonucleotide before it interferes with transformation. At the endonuclease V cleavage site, three nucleotides were preferentially removed from the 3' end and two nucleotides were removed from the 5' end. dI:dT and dI:dC base pairs gave the same results. Caveats include possible interference by Beta protein and by mispaired bases. Thus, oligonucleotide transformation can be used to determine the relative importance of redundant repair pathways, to isolate new DNA repair mutants, and to determine with high precision the sizes of repair tracts in intact cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.