ArticleProceedings of the National Academy of Sciences of the United States of America2007
Regulatory evolution in proteins by turnover and lineage-specific changes of cyclin-dependent kinase consensus sites.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 71 citations in OpenAlex.
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- A functionally divergent intrinsically disordered region underlying the conservation of stochastic signaling.PLoS genetics · 2021Article
- The CRK2-CYC13 complex functions as an S-phase cyclin-dependent kinase to promote DNA replication in Trypanosoma brucei.BMC biology · 2021Article
- The evolutionary scaling of cellular traits imposed by the drift barrier.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Theoretical Framework for Evolutionary Cell Biology.Journal of molecular biology · 2020Review
- Evidence for Stabilizing Selection Driving Mutational Turnover of Short Motifs in the Eukaryotic Complementary Sex Determiner (Csd) Protein.G3 (Bethesda, Md.) · 2018Article
- Most m6A RNA Modifications in Protein-Coding Regions Are Evolutionarily Unconserved and Likely Nonfunctional.Molecular biology and evolution · 2018Article
- Conservation of kinase-phosphorylation site pairings: Evidence for an evolutionarily dynamic phosphoproteome.PloS one · 2018Article
- Co-occurring protein phosphorylation are functionally associated.PLoS computational biology · 2017Article
- Selection maintains signaling function of a highly diverged intrinsically disordered region.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Computational Analysis of the Predicted Evolutionary Conservation of Human Phosphorylation Sites.PloS one · 2016Article
- Motif co-regulation and co-operativity are common mechanisms in transcriptional, post-transcriptional and post-translational regulation.Cell communication and signaling : CCS · 2015Review
- Short linear motifs - ex nihilo evolution of protein regulation.Cell communication and signaling : CCS · 2015Review
- Evolutionary conservation of the CDK targets in eukaryotic DNA replication initiation.Chromosoma · 2015Review
- Prediction of Functionally Important Phospho-Regulatory Events in Xenopus laevis Oocytes.PLoS computational biology · 2015Article
- Bora downregulation results in radioresistance by promoting repair of double strand breaks.PloS one · 2015Article
- Evolutionary constraint and disease associations of post-translational modification sites in human genomes.PLoS genetics · 2015Article
- Detecting functional divergence after gene duplication through evolutionary changes in posttranslational regulatory sequences.PLoS computational biology · 2014Article
- Evolution of bacterial protein-tyrosine kinases and their relaxed specificity toward substrates.Genome biology and evolution · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Evolutionary change in gene regulation is a key mechanism underlying the genetic component of organismal diversity. Here, we study evolution of regulation at the posttranslational level by examining the evolution of cyclin-dependent kinase (CDK) consensus phosphorylation sites in the protein subunits of the pre-replicative complex (RC). The pre-RC, an assembly of proteins formed during an early stage of DNA replication, is believed to be regulated by CDKs throughout the animals and fungi. Interestingly, although orthologous pre-RC components often contain clusters of CDK consensus sites, the positions and numbers of sites do not seem conserved. By analyzing protein sequences from both distantly and closely related species, we confirm that consensus sites can turn over rapidly even when the local cluster of sites is preserved, consistent with the notion that precise positioning of phosphorylation events is not required for regulation. We also identify evolutionary changes in the clusters of sites and further examine one replication protein, Mcm3, where a cluster of consensus sites near a nucleocytoplasmic transport signal is confined to a specific lineage. We show that the presence or absence of the cluster of sites in different species is associated with differential regulation of the transport signal. These findings suggest that the CDK regulation of MCM nuclear localization was acquired in the lineage leading to Saccharomyces cerevisiae after the divergence with Candida albicans. Our results begin to explore the dynamics of regulatory evolution at the posttranslational level and show interesting similarities to recent observations of regulatory evolution at the level of transcription.
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