ArticleCerebrospinal fluid research2007
Evaluation of the IS6110 PCR assay for the rapid diagnosis of tuberculous meningitis.
Article in Cerebrospinal fluid research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 2 of them syntheses that pooled it.
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Who cites it
23 citing papers in PubMed, 2 syntheses or guidelines pooled it, 67 citations in OpenAlex.
- Diagnostic accuracy of GeneXpert in the diagnosis of spinal tuberculosis: A systematic review and meta-analysis.Narra J · 2024Pooled it
- A Systematic Review and Meta-analysis of the Diagnostic Accuracy of Nucleic Acid Amplification Tests for Tuberculous Meningitis.Journal of clinical microbiology · 2019Pooled it
- Optimizing a Conventional Multiplex PCR for Simultaneous Detection of Granulomatous Skin Infection Agents:Journal of tropical medicine · 2026Article
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- Pancreatin-Cetyl Pyridinium Chloride Digestion and Decontamination Method; A Novel, Sensitive, Cost-Effective Method for CulturingMicroorganisms · 2021Article
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- Combined testing for herpes simplex virus and Mycobacterium tuberculosis DNA in cerebrospinal fluid of patients with aseptic meningitis in Burkina Faso, West Africa.Journal of clinical laboratory analysis · 2019Article
- Development of a high sensitivity TaqMan-based PCR assay for the specific detection of Mycobacterium tuberculosis complex in both pulmonary and extrapulmonary specimens.Scientific reports · 2019Article
- The Feasibility of Metagenomic Next-Generation Sequencing to Identify Pathogens Causing Tuberculous Meningitis in Cerebrospinal Fluid.Frontiers in microbiology · 2019Article
- Sensitive detection of the ISRSC advances · 2018Article
- Development and application of a rapid Mycobacterium tuberculosis detection technique using polymerase spiral reaction.Scientific reports · 2018Article
- Thermotropic Liquid Crystal-Assisted Chemical and Biological Sensors.Materials (Basel, Switzerland) · 2017Review
- Prevalence of zoonotic tuberculosis and associated risk factors in Central Indian populations.Journal of epidemiology and global health · 2017Article
- Comparative Diagnostic Utility of IS6110 PCR Assay in CSF and Peripheral Blood Samples of Tuberculous Meningitis Patients: A Pilot Study from Central India.Journal of clinical and diagnostic research : JCDR · 2017Article
- Comparison of amplicor and GeneXpert MTB/RIF tests for diagnosis of tuberculous meningitis.Journal of clinical microbiology · 2014Article
- Rapid detection of Mycobacterium tuberculosis in clinical samples by multiplex polymerase chain reaction (mPCR).World journal of microbiology & biotechnology · 2013Article
- Laboratory investigations on the diagnosis of tuberculosis in the malnourished tribal population of melghat, India.PloS one · 2013Article
- The PCR-Based Diagnosis of Central Nervous System Tuberculosis: Up to Date.Tuberculosis research and treatment · 2012Article
- Article
- Loop-mediated isothermal amplification for rapid and reliable diagnosis of tuberculous meningitis.Journal of clinical microbiology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTuberculous meningitis (TBM) is one of the common clinical manifestations of extra-pulmonary tuberculosis. It is difficult to diagnose due to a lack of rapid, sensitive, and specific tests. Newer methods, which are easy and reliable, are required to diagnose TBM at an early stage. Thus our aim was to evaluate the polymerase chain reaction (PCR) technique, using primers directed against the IS6110 gene, for the detection of Mycobacterium tuberculosis in the CSF, for the diagnosis of TBM patients.
methodsAn in-house IS6110 PCR method using a specific pair of primers designed to amplify the insertion sequence, IS6110, in the M. tuberculosis genome was used to analyze CSF. A total of 80 CSF samples from different groups of patients were studied (confirmed TBM n = 35, clinically suspected TBM n = 16, non-TBM infectious meningitis n = 12, non infectious neurological diseases n = 17).
resultsPCR gave a sensitivity of 91.4% and specificity of 75.9% for the diagnosis of TBM in patients with TBM confirmed by culture. In 16 clinically diagnosed, but unconfirmed, TBM cases PCR was positive in 10 (62.5%) cases. There were seven (24.1%) PCR-positive cases among the 29 patients with non-TBM and non-infectious neurological disease.
conclusionWe conclude that the performance of an in-house IS6110 PCR assay is valuable in the rapid diagnosis of tuberculous meningitis.
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Registered trials
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