ArticleGlycoconjugate journal2008
"Fluorescent glycogen" formation with sensibility for in vivo and in vitro detection.
Article in Glycoconjugate journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 58 citations in OpenAlex.
- Glycogen drives the sensory activation of POMC neurons.Nature metabolism · 2026Article
- Caffeoylquinic Acids From Lonicera japonica Thunb. as Hypoglycemic Agents: Network Pharmacology and Pharmacological Validation.Journal of diabetes research · 2026Article
- Mapping of neurovascular and neurometabolic couplings by multimodal optical imaging.Cerebral cortex (New York, N.Y. : 1991) · 2025Article
- Glycogenesis and glyconeogenesis from glutamine, lactate and glycerol support human macrophage functions.EMBO reports · 2024Article
- Anti-echinococcal effect of metformin in advanced experimental cystic echinococcosis: reprogrammed intermediary carbon metabolism in the parasite.Antimicrobial agents and chemotherapy · 2024Article
- Adjudin improves beta cell maturation, hepatic glucose uptake and glucose homeostasis.Diabetologia · 2024Article
- Harnessing dual-energy CT for glycogen quantification: a phantom analysis.Quantitative imaging in medicine and surgery · 2023Article
- A Micro-Scale Analytical Method for Determining Glycogen Turnover by NMR and FTMS.Metabolites · 2022Article
- KCNH6 Enhanced Hepatic Glucose Metabolism through Mitochondrial CaOxidative medicine and cellular longevity · 2022Article
- Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries.Biotechnology for biofuels · 2021Review
- Visualizing Subcellular Enrichment of Glycogen in Live Cancer Cells by Stimulated Raman Scattering.Analytical chemistry · 2020Article
- Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes.Cellular and molecular neurobiology · 2020Article
- Article
- Revisiting Glycogen in Cancer: A Conspicuous and Targetable Enabler of Malignant Transformation.Frontiers in oncology · 2020Review
- Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.Cell metabolism · 2019Article
- Glycogen metabolism has a key role in the cancer microenvironment and provides new targets for cancer therapy.Journal of molecular medicine (Berlin, Germany) · 2016Review
- Imaging metabolic heterogeneity in cancer.Molecular cancer · 2016Review
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- Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States.PloS one · 2015Article
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There are presently many methods of detecting complex carbohydrates, and particularly glycogen. However most of them require radioisotopes or destruction of the tissue and hydrolysis of glycogen to glucose. Here we present a new method based on the incorporation of 2-NBDG (2-{N-[7-nitrobenz-2-oxa-1, 3-diazol 4-yl] amino}-2-deoxyglucose), a D-glucose fluorescent derivative, into glycogen. Two kinds of approaches were carried out by using Clone 9 rat hepatocytes as a cellular model; (1) Incubation of cell lysates with 2-NBDG, carbohydrate precipitation in filters and measurement of fluorescence in a microplate reader (2) Incubation of living hepatocytes with 2-NBDG and recording of fluorescence images by confocal microscopy. 2-NBDG labeled glycogen in both approaches. We confirmed this fact by comparison to the labeling obtained with a specific monoclonal anti-glycogen antibody. Also drugs that trigger glycogen synthesis or degradation induced an increase or decrease of fluorescence, respectively. This is a simple but efficient method of detecting glycogen with 2-NBDG. It could be used to record changes in glycogen stores in living cells and cell-free systems and opens the prospect of understanding the role of this important energy reserve under various physiological and pathophysiological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.