ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2008
Comparison of seven commercial DNA extraction kits for the recovery of Brucella DNA from spiked human serum samples using real-time PCR.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 85 citations in OpenAlex.
- A critical review of diagnostic methods for camel brucellosis.Veterinary and animal science · 2026Review
- Two novel real-time PCR assays forMicrobiology spectrum · 2026Article
- Brucellosis: Bacteriology, pathogenesis, epidemiology and role of the metallophores in virulence: a review.Frontiers in cellular and infection microbiology · 2025Review
- Investigation of the effects of mir-219-1 gene variants on the development of disease in non-small cell lung cancer patients.African health sciences · 2022Article
- Faradaic-free electrokinetic nucleic acid amplification (E-NAAMP) using localized on-chip high frequency Joule heating.Biomicrofluidics · 2022Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- A robust, hand-powered, instrument-free sample preparation system for point-of-care pathogen detection.Scientific reports · 2019Article
- Extracting the invisible: obtaining high quality DNA is a challenging task in small arthropods.PeerJ · 2019Article
- Optimization of Brucella abortus Protocols for Downstream Molecular Applications.Journal of clinical microbiology · 2018Article
- Detection and identification of Legionella species in hospital water supplies through Polymerase Chain Reaction (16S rRNA).Journal of environmental health science & engineering · 2014Article
- Comparison of DNA extraction kits for detection of Burkholderia pseudomallei in spiked human whole blood using real-time PCR.PloS one · 2013Article
- DNA extract characterization process for microbial detection methods development and validation.BMC research notes · 2012Article
- Challenges of establishing the correct diagnosis of outbreaks of acute febrile illnesses in Africa: the case of a likely Brucella outbreak among nomadic pastoralists, northeast Kenya, March-July 2005.The American journal of tropical medicine and hygiene · 2011Article
- Article
- Same-day detection of Escherichia coli O157:H7 from spinach by using electrochemiluminescent and cytometric bead array biosensors.Applied and environmental microbiology · 2010Article
- Review of detection of Brucella spp. by polymerase chain reaction.Croatian medical journal · 2010Review
- Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR.BMC infectious diseases · 2010Article
- Comparisons of three automated systems for genomic DNA extraction in a clinical diagnostic laboratory.Yonsei medical journal · 2010Article
- The era of molecular and other non-culture-based methods in diagnosis of sepsis.Clinical microbiology reviews · 2010Review
- Evaluation of automated and manual commercial DNA extraction methods for recovery of Brucella DNA from suspensions and spiked swabs.Journal of clinical microbiology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We compared the relative recovery of extraction of bacterial DNA from serum using seven commercial kits (UltraClean DNA BloodSpin Kit, Puregene DNA Purification System, Wizard Genomic DNA Purification Kit, High Pure PCR Template Preparation Kit, GFX Genomic Blood DNA Purification Kit, NucleoSpin Tissue Kit, and QIAamp DNA Blood Mini Kit). Human serum samples were spiked with known concentrations of Brucella melitensis Rev 1; the DNA was extracted and tested in genus-specific LightCycler polymerase chain reaction (PCR). The UltraClean DNA BloodSpin Kit proved to be as sensitive as the QIAamp DNA Blood Mini Kit isolation method and could detect down to 100 fg of DNA, though only the former had no contamination. All the other procedures yielded DNA isolation results that were less sensitive and were always contaminated. Our results show that the UltraClean DNA Blood Spin Kit was the commercially available assay tested that yielded the best sensitivity, purity, and lack of contamination for Brucella DNA isolation from serum.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.