ArticleCurrent genetics2008
Deletion of the cpku80 gene in the chestnut blight fungus, Cryphonectria parasitica, enhances gene disruption efficiency.
Article in Current genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Hypovirus-Induced Phosphorylation of CpIre1 Modulates Unfolded Protein Response and Virulence in Cryphonectria parasitica.Molecular plant pathology · 2026Article
- Genome-Wide Identification and Functional Characterization of the Acyl-CoA Dehydrogenase (ACAD) Family inInternational journal of molecular sciences · 2025Article
- mmBio · 2025Article
- N6-methyladenosine RNA methyltransferase CpMTA1 mediates CpAphA mRNA stability through a YTHDF1-dependent m6A modification in the chestnut blight fungus.PLoS pathogens · 2024Article
- Argonaute-independent, Dicer-dependent antiviral defense against RNA viruses.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Comparative acetylomic analysis reveals differentially acetylated proteins regulating fungal metabolism in hypovirus-infected chestnut blight fungus.Molecular plant pathology · 2023Article
- Applying molecular and genetic methods to trees and their fungal communities.Applied microbiology and biotechnology · 2023Review
- Hypovirus infection induces proliferation and perturbs functions of mitochondria in the chestnut blight fungus.Frontiers in microbiology · 2023Article
- A structural homologue of the plant receptor D14 mediates responses to strigolactones in the fungal phytopathogen Cryphonectria parasitica.The New phytologist · 2022Article
- Bioinoculants-Natural Biological Resources for Sustainable Plant Production.Microorganisms · 2021Review
- Functional Roles of Homologous Recombination and Non-Homologous End Joining in DNA Damage Response and Microevolution inJournal of fungi (Basel, Switzerland) · 2021Article
- Dicer functions transcriptionally and posttranscriptionally in a multilayer antiviral defense.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- CpATG8, a Homolog of Yeast Autophagy Protein ATG8, Is Required for Pathogenesis and Hypovirus Accumulation in the Chest Blight Fungus.Frontiers in cellular and infection microbiology · 2019Article
- Article
- Comparative Methylome Analysis Reveals Perturbation of Host Epigenome in Chestnut Blight Fungus by a Hypovirus.Frontiers in microbiology · 2018Article
- Development of an efficient vector system for gene knock-out and near in-cis gene complementation in the sugarcane smut fungus.Scientific reports · 2017Article
- SAGA complex mediates the transcriptional up-regulation of antiviral RNA silencing.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Engineering super mycovirus donor strains of chestnut blight fungus by systematic disruption of multilocus vic genes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A ku70 null mutant improves gene targeting frequency in the fungal pathogen Verticillium dahliae.World journal of microbiology & biotechnology · 2015Article
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Authors and funding
7 authors.
Funding
Abstract
The chestnut blight fungus, Cryphonectria parasitica, and associated virulence-attenuating hypoviruses have emerged as an important model system for studying molecular mechanisms underlying fungal-plant pathogenic interactions. As more gene sequence information becomes available as a result of C. parasitica express sequence tags (ESTs) and ongoing whole genome sequencing projects, the development of an efficient gene disruption system has become an urgent need for functional genomics studies of this important forestry pathogen. Here, we report the cloning of the C. parasitica gene cpku80 that encodes a key component of the nonhomologous end joining DNA repair pathway and the construction of a corresponding deletion mutant strain. The cpku80 mutant was indistinguishable from the parental wild-type strain EP155 in colony morphology, ability to support hypovirus replication, conidiation and virulence. As predicted, the Deltacpku80 strain did exhibit an increased sensitivity to the mutagen methyl methanesulfonate. A test with three selected genes resulted in a gene disruption efficiency of about 80% for the Deltacpku80 strain, a significant increase over the 2-5% levels of homologous recombination generally observed for the wild-type strain EP155. This efficient homologous recombination system provides a powerful tool for large-scale analysis of gene functions in C. parasitica.
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