ArticleProceedings of the National Academy of Sciences of the United States of America2007
Single-molecule level analysis of the subunit composition of the T cell receptor on live T cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 79 citations in OpenAlex.
- Pre-T cell receptor localization and trafficking are independent of its signaling.The Journal of cell biology · 2023Article
- Pre-organized landscape of T cell surface.Frontiers in immunology · 2023Review
- Surfaces for Study of Receptor Dynamics on T Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Bioengineering tools for probing intracellular events in T lymphocytes.WIREs mechanisms of disease · 2021Review
- The Nanoscopic Organization of Synapse Structures: A Common Basis for Cell Communication.Membranes · 2021Review
- K-Neighbourhood Analysis: A Method for Understanding SMLM Images as Compositions of Local Neighbourhoods.Frontiers in bioinformatics · 2021Article
- T-Cell Receptor Ligands: Every which Way They Can.Biophysical journal · 2020Article
- Single-molecule investigations of T-cell activation.Current opinion in biomedical engineering · 2019Article
- A cell topography-based mechanism for ligand discrimination by the T cell receptor.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Single-Molecule Light-Sheet Imaging of Suspended T Cells.Biophysical journal · 2018Article
- Monomeric TCRs drive T cell antigen recognition.Nature immunology · 2018Article
- Capturing resting T cells: the perils of PLL.Nature immunology · 2018Article
- Membrane Ultrastructure and T Cell Activation.Frontiers in immunology · 2018Review
- Three-Dimensional Super-Resolution in Eukaryotic Cells Using the Double-Helix Point Spread Function.Biophysical journal · 2017Article
- A DNA-Based T Cell Receptor Reveals a Role for Receptor Clustering in Ligand Discrimination.Cell · 2017Article
- Review
- Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Nanoclustering as a dominant feature of plasma membrane organization.Journal of cell science · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
The T cell receptor (TCR) expressed on most T cells is a protein complex consisting of TCRalphabeta heterodimers that bind antigen and cluster of differentiation (CD) 3epsilondelta, epsilongamma, and zetazeta dimers that initiate signaling. A long-standing controversy concerns whether there is one, or more than one, alphabeta heterodimer per complex. We used a form of single-molecule spectroscopy to investigate this question on live T cell hybridomas. The method relies on detecting coincident fluorescence from single molecules labeled with two different fluorophores, as the molecules diffuse through a confocal volume. The fraction of events that are coincident above the statistical background is defined as the "association quotient," Q. In control experiments, Q was significantly higher for cells incubated with wheat germ agglutinin dual-labeled with Alexa488 and Alexa647 than for cells incubated with singly labeled wheat germ agglutinin. Similarly, cells expressing the homodimer, CD28, gave larger values of Q than cells expressing the monomer, CD86, when incubated with mixtures of Alexa488- and Alexa647-labeled antibody Fab fragments. T cell hybridomas incubated with mixtures of anti-TCRbeta Fab fragments labeled with each fluorophore gave a Q value indistinguishable from the Q value for CD86, indicating that the dominant form of the TCR comprises single alphabeta heterodimers. The values of Q obtained for CD86 and the TCR were low but nonzero, suggesting that there is transient or nonrandom confinement, or diffuse clustering of molecules at the T cell surface. This general method for analyzing the subunit composition of protein complexes could be extended to other cell surface or intracellular complexes, and other living cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.