ArticleBMC molecular biology2007
A versatile approach to multiple gene RNA interference using microRNA-based short hairpin RNAs.
Article in BMC molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 61 citations in OpenAlex.
- RNA Interference Applied to Crustacean Aquaculture.Biomolecules · 2024Review
- A lentiviral vector for the production of T cells with an inducible transgene and a constitutively expressed tumour-targeting receptor.Nature biomedical engineering · 2023Article
- Multiple dengue virus serotypes resistant transgenicRNA biology · 2020Article
- A simplified system for the effective expression and delivery of functional mature microRNAs in mammalian cells.Cancer gene therapy · 2020Article
- Antiviral effects against influenza A virus infection by a short hairpin RNA targeting the non-coding terminal region of the viral nucleoprotein gene.The Journal of veterinary medical science · 2019Article
- IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages.Cell reports · 2018Article
- Epstein-Barr Virus (EBV) Tegument Protein BGLF2 Promotes EBV Reactivation through Activation of the p38 Mitogen-Activated Protein Kinase.Journal of virology · 2016Article
- Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana.Plant methods · 2016Article
- shRNA off-target effects in vivo: impaired endogenous siRNA expression and spermatogenic defects.PloS one · 2015Article
- Targeting Myc in KSHV-associated primary effusion lymphoma with BET bromodomain inhibitors.Oncogene · 2014Article
- Inhibition of Bim enhances replication of varicella-zoster virus and delays plaque formation in virus-infected cells.Journal of virology · 2014Article
- Bicistronic gene transfer tools for delivery of miRNAs and protein coding sequences.International journal of molecular sciences · 2013Article
- Acquired MET expression confers resistance to EGFR inhibition in a mouse model of glioblastoma multiforme.Oncogene · 2012Article
- Adaptor protein complex-2 (AP-2) and epsin-1 mediate protease-activated receptor-1 internalization via phosphorylation- and ubiquitination-dependent sorting signals.The Journal of biological chemistry · 2011Article
- Creating a flexible multiple microRNA expression vector by linking precursor microRNAs.Biochemical and biophysical research communications · 2011Article
- A comparison of multiple shRNA expression methods for combinatorial RNAi.Genetic vaccines and therapy · 2011Article
- Modified gateway system for double shRNA expression and Cre/lox based gene expression.BMC biotechnology · 2011Article
- Multiple shRNA combinations for near-complete coverage of all HIV-1 strains.AIDS research and therapy · 2011Article
- Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.PloS one · 2011Article
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Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundEffective and stable knockdown of multiple gene targets by RNA interference is often necessary to overcome isoform redundancy, but it remains a technical challenge when working with intractable cell systems.
resultsWe have developed a flexible platform using RNA polymerase II promoter-driven expression of microRNA-like short hairpin RNAs which permits robust depletion of multiple target genes from a single transcript. Recombination-based subcloning permits expression of multi-shRNA transcripts from a comprehensive range of plasmid or viral vectors. Retroviral delivery of transcripts targeting isoforms of cAMP-dependent protein kinase in the RAW264.7 murine macrophage cell line emphasizes the utility of this approach and provides insight to cAMP-dependent transcription.
conclusionWe demonstrate functional consequences of depleting multiple endogenous target genes using miR-shRNAs, and highlight the versatility of the described vector platform for multiple target gene knockdown in mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.