ArticleCellular microbiology2008
iNOS activity is critical for the clearance of Burkholderia mallei from infected RAW 264.7 murine macrophages.
Article in Cellular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Glanders: Historical military use and potential bioterrorism concern.Open veterinary journal · 2025Review
- Proteomic Analysis of Non-human Primate Peripheral Blood Mononuclear Cells DuringFrontiers in microbiology · 2021Article
- AFrontiers in microbiology · 2020Article
- Transcriptome analysis of human monocytic cells infected with Burkholderia species and exploration of pentraxin-3 as part of the innate immune response against the organisms.BMC medical genomics · 2019Article
- Blowing epithelial cell bubbles with GumB: ShlA-family pore-forming toxins induce blebbing and rapid cellular death in corneal epithelial cells.PLoS pathogens · 2019Article
- Relative susceptibility of airway organisms to antimicrobial effects of nitric oxide.International forum of allergy & rhinology · 2017Article
- Characterization of in vitro phenotypes of Burkholderia pseudomallei and Burkholderia mallei strains potentially associated with persistent infection in mice.Archives of microbiology · 2017Article
- pH Alkalinization by Chloroquine Suppresses Pathogenic Burkholderia Type 6 Secretion System 1 and Multinucleated Giant Cells.Infection and immunity · 2017Article
- Phenotypic Characterization of a Novel Virulence-Factor Deletion Strain of Burkholderia mallei That Provides Partial Protection against Inhalational Glanders in Mice.Frontiers in cellular and infection microbiology · 2016Article
- Characterization of the murine macrophage response to infection with virulent and avirulent Burkholderia species.BMC microbiology · 2015Article
- A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection.Frontiers in microbiology · 2015Article
- A high-content imaging assay for the quantification of the Burkholderia pseudomallei induced multinucleated giant cell (MNGC) phenotype in murine macrophages.BMC microbiology · 2014Article
- Control of intracellular Francisella tularensis by different cell types and the role of nitric oxide.Journal of immunology research · 2014Article
- Article
- Structural and functional characterization of the nitrite channel NirC from Salmonella typhimurium.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The formate channel FocA exports the products of mixed-acid fermentation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Nitric oxide-dependent killing of aerobic, anaerobic and persistent Burkholderia pseudomallei.Nitric oxide : biology and chemistry · 2012Article
- Burkholderia mallei and Burkholderia pseudomallei stimulate differential inflammatory responses from human alveolar type II cells (ATII) and macrophages.Frontiers in cellular and infection microbiology · 2012Article
- The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei.Infection and immunity · 2011Article
- Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages.Infection and immunity · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Burkholderia mallei is a facultative intracellular pathogen that can cause fatal disease in animals and humans. To better understand the role of phagocytic cells in the control of infections caused by this organism, studies were initiated to examine the interactions of B. mallei with RAW 264.7 murine macrophages. Utilizing modified kanamycin-protection assays, B. mallei was shown to survive and replicate in RAW 264.7 cells infected at multiplicities of infection (moi) of < or = 1. In contrast, the organism was efficiently cleared by the macrophages when infected at an moi of 10. Interestingly, studies demonstrated that the monolayers only produced high levels of TNF-alpha, IL-6, IL-10, GM-CSF, RANTES and IFN-beta when infected at an moi of 10. In addition, nitric oxide assays and inducible nitric oxide synthase (iNOS) immunoblot analyses revealed a strong correlation between iNOS activity and clearance of B. mallei from RAW 264.7 cells. Furthermore, treatment of activated macrophages with the iNOS inhibitor, aminoguanidine, inhibited clearance of B. mallei from infected monolayers. Based upon these results, it appears that moi significantly influence the outcome of interactions between B. mallei and murine macrophages and that iNOS activity is critical for the clearance of B. mallei from activated RAW 264.7 cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.