ArticleTransgenic research2008
Lung specific expression of a human mutant p53 affects cell proliferation in transgenic mice.
Article in Transgenic research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Gain of function mutant p53 protein activates AKT through the Rac1 signaling to promote tumorigenesis.Cell cycle (Georgetown, Tex.) · 2020Article
- Gain-of-function mutant p53 activates small GTPase Rac1 through SUMOylation to promote tumor progression.Genes & development · 2017Article
- Aberrant Adult Neurogenesis in the Subventricular Zone-Rostral Migratory Stream-Olfactory Bulb System Following Subchronic Manganese Exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- A novel mutant p53 binding partner BAG5 stabilizes mutant p53 and promotes mutant p53 GOFs in tumorigenesis.Cell discovery · 2016Article
- Mutant p53 promotes ovarian cancer cell adhesion to mesothelial cells via integrin β4 and Akt signals.Scientific reports · 2015Article
- Spliced MDM2 isoforms promote mutant p53 accumulation and gain-of-function in tumorigenesis.Nature communications · 2013Article
- Upregulation of the mitochondrial transport protein, Tim50, by mutant p53 contributes to cell growth and chemoresistance.Archives of biochemistry and biophysics · 2011Article
- MicroRNA-34a is an important component of PRIMA-1-induced apoptotic network in human lung cancer cells.International journal of cancer · 2010Article
- Mutant p53 gain-of-function in cancer.Cold Spring Harbor perspectives in biology · 2010Review
- Cimiside E arrests cell cycle and induces cell apoptosis in gastric cancer cells.Archives of pharmacal research · 2009Article
- Expression of a mutant p53 results in an age-related demographic shift in spontaneous lung tumor formation in transgenic mice.PloS one · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The human mutant p53(273H) has been shown in vitro to have both dominant-negative and gain-of-function properties, as well as to retain partial DNA-binding and transcriptional activation functions. We have developed a line of transgenic mice in which the human mutant p53(273H) is expressed in a lung specific manner (p53 (+/+/TG)). Crossing of the transgenic mice with p53 knockout mice led to generate mice with various genetic backgrounds. To evaluate the influence of p53 mutants in cell proliferation in mice lung tissue, we analyzed cell proliferation rate by Bromodeoxyuridine (BrdU) labeling and by expression of proliferating cell nuclear antigen (PCNA). BrdU analysis showed a 3.7-fold increase in the number of BrdU positive cells in the (p53 (-/+/TG)) mice compared to the (p53 (-/+)) mice, whereas no difference was observed in proliferation rate in the p53 (-/-/TG) lungs as compared to p53 (-/-) lungs. After the mice were treated with gamma-irradiation, BrdU positive cells were absent from both the p53 (-/+/TG) and p53 (-/+) mice, whereas a decrease in the rate of cell proliferation occurred in p53 (-/-/TG) lungs as compared to p53 (-/-) lungs. Real time PCR results indicated that the p53(273H) mutant did not retain the function to activate expression of p21 (WAF1/CIP1) in the transgenic mice. The above results indicate that overexpression of the human mutant p53(273H) in vivo results in an increase in basal proliferation rate which requires the presence of wild type p53. Mutant p53(273H) may affect cell proliferation by interrupting murine endogenous p53 function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.