Evidence map›Paper›PMID 17968323›Full record

ArticleOncogene2008

MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer.

I A Asangani, S A K Rasheed, D A Nikolova, J H Leupold, N H Colburn, S Post, H Allgayer

Abstract read
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In one paragraph

Article in Oncogene, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1,007 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1,007citing papers in PubMed, 4 pooled it
43.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1,007 citing papers in PubMed, 4 syntheses or guidelines pooled it, 1,881 citations in OpenAlex.

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947 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

I A AsanganiDepartment of Experimental Surgery and Molecular Oncology of Solid Tumors, Medical Faculty Mannheim, University of Heidelberg, Germany.
S A K Rasheed
D A Nikolova
J H Leupold
N H Colburn
S Post
H Allgayer
University Hospital Heidelberg · DEHeidelberg University · DENational Institutes of Health · US

Funding

The Role of Pdcd4 in Translation, Tumorigenesis and Tumor ProgressionZIABC010026 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI COLBURN, NANCY H. · 2009 to 2013
$2.7M
The Role of Pdcd4 in Translation, Tumorigenesis and Tumor ProgressionZ01BC010026 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI COLBURN, NANCY H. · 1996 to 2008
$1.5M
6 · The paper itself

Abstract

Tumor-suppressor Pdcd4 inhibits transformation and invasion and is downregulated in cancers. So far, it has not been studied as to whether miRNAs, suppressing target expression by binding to the 3'-UTR, regulate Pdcd4 or invasion. The present study was conducted to investigate the regulation of Pdcd4, and invasion/intra-vasation, by miRNAs. A bioinformatics search revealed a conserved target-site for miR-21 within the Pdcd4-3'-UTR at 228-249 nt. In 10 colorectal cell lines, an inverse correlation of miR-21 and Pdcd4-protein was observed. Transfection of Colo206f-cells with miR-21 significantly suppressed a luciferase-reporter containing the Pdcd4-3'-UTR, whereas transfection of RKO with anti-miR-21 increased activity of this construct. This was abolished when a construct mutated at the miR-21/nt228-249 target site was used instead. Anti-miR-21-transfected RKO cells showed an increase of Pdcd4-protein and reduced invasion. Moreover, these cells showed reduced intra-vasation and lung metastasis in a chicken-embryo-metastasis assay. In contrast, overexpression of miR-21 in Colo206f significantly reduced Pdcd4-protein amounts and increased invasion, while Pdcd4-mRNA was unaltered. Resected normal/tumor tissues of 22 colorectal cancer patients demonstrated an inverse correlation between miR-21 and Pdcd4-protein. This is the first study to show that Pdcd4 is negatively regulated by miR-21. Furthermore, it is the first report to demonstrate that miR-21 induces invasion/intravasation/metastasis.

Indexed as

RNA InterferenceAdenocarcinomaAnimalsApoptosis Regulatory ProteinsBase SequenceCaco-2 CellsChick EmbryoColorectal NeoplasmsDown-RegulationGene Expression Regulation, NeoplasticGenes, Tumor SuppressorHCT116 CellsHT29 CellsHumansMicroRNAsMolecular Sequence DataApoptosis Regulatory ProteinsMicroRNAsMIRN21 microRNA, humanPDCD4 protein, humanRNA-Binding Proteins

Identifiers

PMID17968323
OpenAlexW2050079849

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.