ArticleProceedings of the National Academy of Sciences of the United States of America2007
Quantum dots provide an optical signal specific to full collapse fusion of synaptic vesicles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 92 citations in OpenAlex.
- Exposure to Nanoplastics Disrupts Neurotransmitter Release in Rat Hippocampal Neurons.Environment & health (Washington, D.C.) · 2026Article
- Synaptic Vesicle Recycling Through the Lens of Ultrafast Endocytosis.Annual review of neuroscience · 2025Review
- Altered exocytosis of inhibitory synaptic vesicles at single presynaptic terminals of cultured striatal neurons in a knock-in mouse model of Huntington's disease.Frontiers in molecular neuroscience · 2023Article
- Calcineurin and huntingtin form a calcium-sensing machinery that directs neurotrophic signals to the nucleus.Science advances · 2022Article
- Methods of measuring presynaptic function with fluorescence probes.Applied microscopy · 2021Review
- Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Synaptic vesicle traffic is supported by transient actin filaments and regulated by PKA and NO.Nature communications · 2020Article
- Lighting up the brain: genetically encoded fluorescent sensors for imaging neurotransmitters and neuromodulators.Current opinion in neurobiology · 2018Review
- Membrane cholesterol mediates the cellular effects of monolayer graphene substrates.Nature communications · 2018Article
- Amperometry methods for monitoring vesicular quantal size and regulation of exocytosis release.Pflugers Archiv : European journal of physiology · 2018Review
- Altered Synaptic Vesicle Release and CaFrontiers in molecular neuroscience · 2018Article
- Presynaptic morphology and vesicular composition determine vesicle dynamics in mouse central synapses.eLife · 2017Article
- Flash-and-Freeze: Coordinating Optogenetic Stimulation with Rapid Freezing to Visualize Membrane Dynamics at Synapses with Millisecond Resolution.Frontiers in synaptic neuroscience · 2016Article
- Activity-dependent BDNF release via endocytic pathways is regulated by synaptotagmin-6 and complexin.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Presynaptic active zones in invertebrates and vertebrates.EMBO reports · 2015Review
- Synaptic vesicle recycling: steps and principles.The EMBO journal · 2014Review
- Article
- Ultrafast endocytosis at mouse hippocampal synapses.Nature · 2013Article
- Article
- Amperometric resolution of a prespike stammer and evoked phases of fast release from retinal bipolar cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Synaptic vesicles are responsible for releasing neurotransmitters and are thus essential to brain function. The classical mode of vesicle recycling includes full collapse of the vesicle into the plasma membrane and clathrin-mediated regeneration of a new vesicle. In contrast, a nonclassical mode known as "kiss-and-run" features fusion by a transient fusion pore without complete loss of vesicle identity and offers possible advantages for increasing the throughput of neurotransmission. Studies of vesicular traffic have benefited greatly from fluorescent probes like FM dyes and synaptopHluorin. However, intrinsic properties of these probes limit their ability to provide a simple and precise distinction between classical and nonclassical modes. Here we report a novel optical probe specific to full collapse fusion, capitalizing on the size and superior photo-properties of photoluminescent quantum dots (Qdots). Qdots with exposed carboxyl groups were readily taken up by synaptic vesicles in an activity-, Ca(2+)-, and clathrin-dependent manner. Electron microscopy showed that Qdots were harbored within individual vesicles in a 1:1 ratio. The release of Qdots was activity- and Ca(2+)-dependent, similar to FM dyes. As artificial cargo, approximately 15 nm in diameter, Qdots will not escape vesicles during kiss-and-run but only with full collapse fusion. Strikingly, Qdots unloaded with kinetics substantially slower than destaining of FM dye, indicating that full-collapse fusion contributed only a fraction of all fusion events. As a full-collapse-fusion-responsive reporter, Qdots will likely promote better understanding of vesicle recycling at small CNS nerve terminals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.