ArticleAntimicrobial agents and chemotherapy2008
Toluidine blue-mediated photodynamic effects on staphylococcal biofilms.
Article in Antimicrobial agents and chemotherapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 180 citations in OpenAlex.
- Photodynamic inactivation of microorganisms present on complete dentures. A clinical investigation. Photodynamic disinfection of complete dentures.Lasers in medical science · 2012Trial
- Enhanced photodynamic disruption of multidrug-resistantBiochemistry and biophysics reports · 2025Article
- Antimicrobial photodynamic therapy against multidrug-resistant Staphylococcus aureus using aluminum chloride phthalocyanine nanoemulsion: in vitro and in vivo studies.Folia microbiologica · 2025Article
- Rational Design and Evaluation of Photoactive Molecularly Imprinted Nanoparticles for Tetracycline Degradation Under Visible Light.ACS omega · 2024Article
- Article
- Light-Driven Tetra- and Octa-β-substituted Cationic Zinc(II) Phthalocyanines for EradicatingInternational journal of molecular sciences · 2023Article
- Photodynamic toluidine blue-gold nanoconjugates as a novel therapeutic forRSC advances · 2023Article
- The application of selenium nanoparticles for enhancing the efficacy of photodynamic inactivation of planktonic communities and the biofilm of Streptococcus mutans.BMC research notes · 2022Article
- Evaluation of antimicrobial photodynamic therapy with acidic methylene blue for the treatment of experimental periodontitis.PloS one · 2022Article
- A nano phototheranostic approach of toluidine blue conjugated gold silver core shells mediated photodynamic therapy to treat diabetic foot ulcer.Scientific reports · 2021Article
- Phototherapy and optical waveguides for the treatment of infection.Advanced drug delivery reviews · 2021Review
- Antibacterial Effects of Bicarbonate in Media Modified to Mimic Cystic Fibrosis Sputum.International journal of molecular sciences · 2020Article
- The efficacy of photodynamic inactivation with laser diode onInfectious disease reports · 2020Article
- Synthesis and In Vitro Photodynamic Activity of Cationic Boron Dipyrromethene-Based Photosensitizers against Methicillin-ResistantBiomedicines · 2020Article
- Evaluation of Toluidine Blue-Mediated Photodynamic Therapy for Experimental Bacterial Keratitis in Rabbits.Translational vision science & technology · 2020Article
- The Open Challenge ofFrontiers in bioengineering and biotechnology · 2020Review
- Photodynamic inactivation diminishes quorum sensing-mediated virulence factor production and biofilm formation of Serratia marcescens.World journal of microbiology & biotechnology · 2019Article
- Antimicrobial Photodynamic Therapy Protocols onBioengineering (Basel, Switzerland) · 2019Article
- Nonconventional Therapeutics againstMicrobiology spectrum · 2018Article
- An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration of biofilms that protect the organisms from immune system clearance and to increased resistance to phagocytosis and antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for the inactivation of bacteria in biofilms. In this study, we have investigated the effect of the photodynamic action of toluidine blue O (TBO) on the viability and structure of biofilms of Staphylococcus epidermidis and of a methicillin-resistant Staphylococcus aureus strain. Significant inactivation of cells was observed when staphylococcal biofilms were exposed to TBO and laser simultaneously. The effect was found to be light dose dependent. Confocal laser scanning microscopic study suggested damage to bacterial cell membranes in photodynamically treated biofilms. In addition, scanning electron microscopy provided direct evidence for the disruption of biofilm structure and a decrease in cell numbers in photodynamically treated biofilms. Furthermore, the treatment of biofilms with tetrasodium EDTA followed by PDT enhanced the photodynamic efficacy of TBO in S. epidermidis, but not in S. aureus, biofilms. The results suggest that photodynamic treatment may be a useful approach for the inactivation of staphylococcal biofilms adhering to solid surfaces of medical implants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.