ArticleMolecular and cellular biology2008
Establishment of a novel in vivo sex-specific splicing assay system to identify a trans-acting factor that negatively regulates splicing of Bombyx mori dsx female exons.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 86 citations in OpenAlex.
- The complete sequence of the silkworm W chromosome uncovers its rapid evolution by large-scale duplications/deletions and translocation of W-linked genes.G3 (Bethesda, Md.) · 2026Article
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- Molecular mechanisms of sex determination in Lepidoptera: current status and perspectives.Insect science · 2026Review
- The Masc-PSI complex directly induces male-type doublesex splicing in silkworms.Communications biology · 2025Article
- Understanding the genetics of sex determination in insects and its relevance to genetic pest management.Insect molecular biology · 2025Review
- Theme and variation in the evolution of insect sex determination.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2023Review
- Cell-based analysis reveals that sex-determining gene signals inPNAS nexus · 2023Article
- A database of crop pest cell lines.In vitro cellular & developmental biology. Animal · 2022Article
- Identification and Characterization of the Masculinizing Function of theInternational journal of molecular sciences · 2021Article
- femaleless Controls Sex Determination and Dosage Compensation Pathways in Females of Anopheles Mosquitoes.Current biology : CB · 2021Article
- The Sex Determination Cascade in the Silkworm.Genes · 2021Review
- Transgenic and knockout analyses of Masculinizer and doublesex illuminated the unique functions of doublesex in germ cell sexual development of the silkworm, Bombyx mori.BMC developmental biology · 2020Article
- Establishment and characterization of novel cell lines derived from six lepidopteran insects collected in the field.In vitro cellular & developmental biology. Animal · 2020Article
- Spliceosomal Protein GeneInternational journal of molecular sciences · 2020Article
- Alternative splicing regulation of doublesex gene by RNA-binding proteins in the silkworm Bombyx mori.RNA biology · 2019Article
- P-element Somatic Inhibitor Protein Binding a Target Sequence inInternational journal of molecular sciences · 2019Article
- Unique sex determination system in the silkworm, Bombyx mori: current status and beyond.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2018Review
- Article
- Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination.PLoS genetics · 2017Article
- Transgenic Expression of the piRNA-Resistant Masculinizer Gene Induces Female-Specific Lethality and Partial Female-to-Male Sex Reversal in the Silkworm, Bombyx mori.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Bombyx mori homolog of doublesex, Bmdsx, plays an essential role in silkworm sexual development. Exons 3 and 4 of Bmdsx pre-mRNA are specifically excluded in males. To explore how this occurs, we developed a novel in vivo sex-specific splicing assay system using sexually differentiated cultured cells. A series of mutation analyses using a Bmdsx minigene with this in vivo splicing assay system identified three distinct sequences (CE1, CE2, and CE3) positioned in exon 4 as exonic splicing silencers responsible for male-specific splicing. Gel shift analysis showed that CE1 binds to a nuclear protein from male cells but not that from female cells. Mutation of UAA repeats within CE1 inhibited the binding of the nuclear protein to the RNA and caused female-specific splicing in male cells. We have identified BmPSI, a Bombyx homolog of P-element somatic inhibitor (PSI), as the nuclear factor that specifically binds CE1. Down-regulation of endogenous BmPSI by RNA interference significantly increased female-specific splicing in male cells. This is the first report of a PSI homolog implicated in the regulated sex-specific splicing of dsx pre-mRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.