ArticleInfection and immunity2008
A LysR-type transcriptional regulator in Burkholderia cenocepacia influences colony morphology and virulence.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 66 citations in OpenAlex.
- Quorum sensing in theApplied and environmental microbiology · 2026Review
- The Intricate Nonribosomal Assembly of a Potent Antifungal Lipopeptide from theJournal of the American Chemical Society · 2025Article
- Colony morphotype variation inJournal of bacteriology · 2025Review
- Quorum sensing and DNA methylation play active roles in clinicalJournal of bacteriology · 2025Article
- Catabolite control protein C contributes to virulence and hydrogen peroxide-induced oxidative stress responses inFrontiers in microbiology · 2024Article
- Membrane-enclosedApplied and environmental microbiology · 2023Article
- A LysR Transcriptional Regulator Manipulates Macrophage Autophagy Flux DuringFrontiers in cellular and infection microbiology · 2022Article
- Recent Advances in the Mechanisms and Regulation of QS in Dairy Spoilage byFoods (Basel, Switzerland) · 2021Review
- A LysR Family Transcriptional Regulator Modulates Burkholderia cenocepacia Biofilm Formation and Protease Production.Applied and environmental microbiology · 2021Article
- Genome-Scale Metabolic Model ofFrontiers in genetics · 2020Article
- Biofilm-Constructing Variants of Paraburkholderia phytofirmans PsJN Outcompete the Wild-Type Form in Free-Living and Static Conditions but NotApplied and environmental microbiology · 2019Article
- Phenotypic and Genomic Analyses of Burkholderia stabilis Clinical Contamination, Switzerland.Emerging infectious diseases · 2019Article
- Metatranscriptomic Analysis of the Bacterial SymbiontLife (Basel, Switzerland) · 2019Article
- Article
- The Mla Pathway Plays an Essential Role in the Intrinsic Resistance of Burkholderia cepacia Complex Species to Antimicrobials and Host Innate Components.Journal of bacteriology · 2018Article
- The OmpR Regulator of Burkholderia multivorans Controls Mucoid-to-Nonmucoid Transition and Other Cell Envelope Properties Associated with Persistence in the Cystic Fibrosis Lung.Journal of bacteriology · 2018Article
- Pseudomonas aeruginosa-Derived Rhamnolipids and Other Detergents Modulate Colony Morphotype and Motility in the Burkholderia cepacia Complex.Journal of bacteriology · 2017Article
- Characterization of in vitro phenotypes of Burkholderia pseudomallei and Burkholderia mallei strains potentially associated with persistent infection in mice.Archives of microbiology · 2017Article
- Review
- Comparative Genomics ofFrontiers in microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia cenocepacia strain K56-2 typically has rough colony morphology on agar medium; however, shiny colony variants (shv) can appear spontaneously. These shv all had a minimum of 50% reduction in biomass formation and were generally avirulent in an alfalfa seedling infection model. Three shv-K56-2 S15, K56-2 S76, and K56-2 S86-were analyzed for virulence in a chronic agar bead model of respiratory infection and, although all shv were able to establish chronic infection, they produced significantly less lung histopathology than the rough K56-2. Transmission electron microscopy revealed that an extracellular matrix surrounding bacterial cells was absent or reduced in the shv compared to the rough wild type. Transposon mutagenesis was performed on the rough wild-type strain and a mutant with an insertion upstream of ORF BCAS0225, coding for a putative LysR-type regulator, exhibited shiny colony morphology, reduced biofilm production, increased N-acyl homoserine lactone production, and avirulence in alfalfa. The rough parental colony morphotype, biofilm formation, and virulence in alfalfa were restored by providing BCAS0225 in trans in the BCAS0225::pGSVTp-luxCDABF mutant. Introduction of BCAS0225 restored the rough morphotype in several shv which were determined to have spontaneous mutations in this gene. In the present study, we show that the conversion from rough wild type to shv in B. cenocepacia correlates with reduced biofilm formation and virulence, and we determined that BCAS0225 is one gene involved in the regulation of these phenotypes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.