ArticlePLoS genetics2007
The essential role of Drosophila HIRA for de novo assembly of paternal chromatin at fertilization.
Article in PLoS genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed, 129 citations in OpenAlex.
- Developmental stage dominates cell-type identity and reveals a chromatin regulatory function for Rad50 in Drosophila.Nucleic acids research · 2026Article
- Temporal transcriptional regulation of mitochondrial morphology primes activity-dependent circuit connectivity.Nature communications · 2025Article
- Mitofusin 1 Drives Preimplantation Development by Enhancing Chromatin Incorporation of Histone H3.3.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- HIRA and dPCIF1 coordinately establish totipotent chromatin and control orderly ZGA inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Epigenetic inheritance and gene expression regulation in early Drosophila embryos.EMBO reports · 2024Review
- Multiple Roles of dXNP and dADD1-International journal of molecular sciences · 2023Review
- Ability of a selfish B chromosome to evade genome elimination in the jewel wasp, Nasonia vitripennis.Heredity · 2023Article
- The histone chaperone NASP maintains H3-H4 reservoirs in the early Drosophila embryo.PLoS genetics · 2023Article
- The Cif proteins from Wolbachia prophage WO modify sperm genome integrity to establish cytoplasmic incompatibility.PLoS biology · 2022Article
- Chromatin mutations in pediatric high grade gliomas.Frontiers in oncology · 2022Review
- Histone Chaperones as Cardinal Players in Development.Frontiers in cell and developmental biology · 2022Review
- The epigenetic origin of life history transitions in plants and algae.Plant reproduction · 2021Review
- CHD1 controls H3.3 incorporation in adult brain chromatin to maintain metabolic homeostasis and normal lifespan.Cell reports · 2021Article
- Gametic specialization of centromeric histone paralogs inLife science alliance · 2021Article
- Distinct spermiogenic phenotypes underlie sperm elimination in the Segregation Distorter meiotic drive system.PLoS genetics · 2021Article
- Histone variant H3.3 residue S31 is essential for Xenopus gastrulation regardless of the deposition pathway.Nature communications · 2020Article
- The HIRA histone chaperone complex subunit UBN1 harbors H3/H4- and DNA-binding activity.The Journal of biological chemistry · 2019Article
- Lysine 27 of replication-independent histone H3.3 is required for Polycomb target gene silencing but not for gene activation.PLoS genetics · 2019Article
- ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization.Epigenetics & chromatin · 2018Article
- Single Nucleotide Polymorphisms in the HIRA Gene Affect Litter Size in Small Tail Han Sheep.Animals : an open access journal from MDPI · 2018Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many animal species, the sperm DNA is packaged with male germ line--specific chromosomal proteins, including protamines. At fertilization, these non-histone proteins are removed from the decondensing sperm nucleus and replaced with maternally provided histones to form the DNA replication competent male pronucleus. By studying a point mutant allele of the Drosophila Hira gene, we previously showed that HIRA, a conserved replication-independent chromatin assembly factor, was essential for the assembly of paternal chromatin at fertilization. HIRA permits the specific assembly of nucleosomes containing the histone H3.3 variant on the decondensing male pronucleus. We report here the analysis of a new mutant allele of Drosophila Hira that was generated by homologous recombination. Surprisingly, phenotypic analysis of this loss of function allele revealed that the only essential function of HIRA is the assembly of paternal chromatin during male pronucleus formation. This HIRA-dependent assembly of H3.3 nucleosomes on paternal DNA does not require the histone chaperone ASF1. Moreover, analysis of this mutant established that protamines are correctly removed at fertilization in the absence of HIRA, thus demonstrating that protamine removal and histone deposition are two functionally distinct processes. Finally, we showed that H3.3 deposition is apparently not affected in Hira mutant embryos and adults, suggesting that different chromatin assembly machineries could deposit this histone variant.
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