ArticleMarine biotechnology (New York, N.Y.)
Silencing VP28 gene of white spot syndrome virus of shrimp by bacterially expressed dsRNA.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 76 citations in OpenAlex.
- RNA Interference Applied to Crustacean Aquaculture.Biomolecules · 2024Review
- Transgenic East African Highland Banana Plants Are Protected againstInternational journal of molecular sciences · 2023Article
- Circulating Phylotypes of White Spot Syndrome Virus in Bangladesh and Their Virulence.Microorganisms · 2022Article
- First Evidence of Feeding-Induced RNAi in Banana Weevil via Exogenous Application of dsRNA.Insects · 2021Article
- Feasibility Study on the Use of Fly Maggots (Life (Basel, Switzerland) · 2021Article
- Feeding exogenous dsRNA interferes with endogenous sRNA accumulation in Paramecium.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Article
- A new age in AquaMedicine: unconventional approach in studying aquatic diseases.BMC veterinary research · 2018Review
- The high-throughput production of dsRNA against sacbrood virus for use in the honey bee Apis cerana (Hymenoptera: Apidae).Virus genes · 2016Article
- VP24 Is a Chitin-Binding Protein Involved in White Spot Syndrome Virus Infection.Journal of virology · 2016Article
- Inactivated E. coli transformed with plasmids that produce dsRNA against infectious salmon anemia virus hemagglutinin show antiviral activity when added to infected ASK cells.Frontiers in microbiology · 2015Article
- In vitro inhibition of Cyprinid herpesvirus-3 replication by RNAi.Journal of virological methods · 2014Article
- Protection of shrimp Penaeus monodon from WSSV infection using antisense constructs.Marine biotechnology (New York, N.Y.) · 2014Article
- Transcriptome analysis on Chinese shrimp Fenneropenaeus chinensis during WSSV acute infection.PloS one · 2013Article
- Article
- Uncovering the mechanisms of shrimp innate immune response by RNA interference.Marine biotechnology (New York, N.Y.) · 2011Review
- Tsetse salivary gland hypertrophy virus: hope or hindrance for tsetse control?PLoS neglected tropical diseases · 2011Review
- Protection of Penaeus monodon from Infection of White spot syndrome virus by DNA Construct Expressing Long Hairpin-RNA Against ICP11 Gene.Indian journal of virology : an official organ of Indian Virological Society · 2010Article
- Application of nucleic-acid-based therapeutics for viral infections in shrimp aquaculture.Marine biotechnology (New York, N.Y.)Review
- Oral administration of bacterially expressed VP28dsRNA to protect Penaeus monodon from white spot syndrome virus.Marine biotechnology (New York, N.Y.)Article
- White spot syndrome virus: an overview on an emergent concern.Veterinary researchArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in vivo expression system to produce large amounts of virus-derived dsRNAs in bacteria to provide a practical control of white spot syndrome virus (WSSV) in shrimp was developed. The bacterially synthesized dsRNA specific to VP28 gene of WSSV promoted gene-specific interference with the WSSV infection in shrimp. Virus infectivity was significantly reduced in WSSV-challenged shrimp injected with VP28-dsRNA and 100% survival was recorded. The inhibition of the expression of WSSV VP28 gene in experimentally challenged animals by VP28-dsRNA was confirmed by RT-PCR and Western blot analyses. Furthermore, we have demonstrated the efficacy of bacterially expressed VP28-dsRNA to silence VP28 gene expression in SISK cell line transfected with eukaryotic expression vector (pcDNA3.1) inserted with VP28 gene of WSSV. The expression level of VP28 gene in SISK cells was determined by fluorescent microscopy and ELISA. The results showed that the expression was significantly reduced in cells transfected with VP28dsRNA, whereas the cells transected with pcDNA-VP28 alone showed higher expression. The in vivo production of dsRNA using prokaryotic expression system could be an alternative to in vitro method for large-scale production of dsRNA corresponding to VP28 gene of WSSV for practical application to control the WSSV in shrimp farming.
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