ArticleNature chemical biology2007
Dissecting metal ion-dependent folding and catalysis of a single DNAzyme.
Article in Nature chemical biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- The interplay between circadian rhythms and aging: molecular mechanisms and therapeutic strategies.Biogerontology · 2025Review
- Signal Transduction Strategies for DNAzyme-Based Sensing and Imaging of Metal Ions in Cells andChemical & biomedical imaging · 2025Review
- Article
- Panoramic lead-immune system interactome reveals diversified mechanisms of immunotoxicity upon chronic lead exposure.Cell biology and toxicology · 2025Article
- The dynamic world of the 8-17 DNAzyme.Nature communications · 2024Article
- The Programmable Catalytic Core of 8-17 DNAzymes.Molecules (Basel, Switzerland) · 2024Article
- The 8-17 DNAzyme can operate in a single active structure regardless of metal ion cofactor.Nature communications · 2024Article
- Improving the Sensitivity of a Mn(II)-Specific DNAzyme for Cellular Imaging Sensor through Sequence Mutations.Analytical chemistry · 2024Article
- Development of 8-17 XNAzymes that are functional in cells.Chemical science · 2023Article
- Single-Molecule Study of DNAzyme Reveals Its Intrinsic Conformational Dynamics.International journal of molecular sciences · 2023Article
- Overcoming Major Barriers to Developing Successful Sensors for Practical Applications Using Functional Nucleic Acids.Annual review of analytical chemistry (Palo Alto, Calif.) · 2022Review
- DNA-Based Biosensors for the Biochemical Analysis: A Review.Biosensors · 2022Review
- DNAzyme Sensor Uses Chemiluminescence Resonance Energy Transfer for Rapid, Portable, and Ratiometric Detection of Metal Ions.Analytical chemistry · 2021Article
- A GATA3 Targeting Nucleic Acid Nanocapsule forACS nano · 2021Article
- Wavelength-Selective Activation of Photocaged DNAzymes for Metal Ion Sensing in Live Cells.ACS omega · 2021Article
- Catalytic Nucleic Acids: Biochemistry, Chemical Biology, Biosensors, and Nanotechnology.iScience · 2020Review
- DNAzymes as Activity-Based Sensors for Metal Ions: Recent Applications, Demonstrated Advantages, Current Challenges, and Future Directions.Accounts of chemical research · 2019Review
- Dynamical ensemble of the active state and transition state mimic for the RNA-cleaving 8-17 DNAzyme in solution.Nucleic acids research · 2019Article
- Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells.Molecules (Basel, Switzerland) · 2019Review
- Misfolding of a DNAzyme for ultrahigh sodium selectivity over potassium.Nucleic acids research · 2018Article
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5 authors.
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Abstract
Protein metalloenzymes use various modes for functions for which metal-dependent global conformational change is required in some cases but not in others. In contrast, most ribozymes require a global folding that almost always precedes enzyme reactions. Herein we studied metal-dependent folding and cleavage activity of the 8-17 DNAzyme using single-molecule fluorescence resonance energy transfer. Addition of Zn2+ and Mg2+ induced folding of the DNAzyme into a more compact structure followed by a cleavage reaction, which suggests that the DNAzyme may require metal-dependent global folding for activation. In the presence of Pb2+, however, the cleavage reaction occurred without a precedent folding step, which suggests that the DNAzyme may be prearranged to accept Pb2+ for the activity. Neither ligation reaction of the cleaved substrates nor dynamic changes between folded and unfolded states was observed. These features may contribute to the unusually fast Pb2+-dependent reaction of the DNAzyme. These results suggest that DNAzymes can use all modes of activation that metalloproteins use.
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