ArticleThe Plant cell2007
The catalytically active tyrosine residues of both SPO11-1 and SPO11-2 are required for meiotic double-strand break induction in Arabidopsis.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
81 citing papers in PubMed, 159 citations in OpenAlex.
- Crossover control: A key to unlocking genetic diversity in plant breeding.Journal of integrative plant biology · 2026Review
- Sex distorter male drive for resistance-resilient population control of the human malaria vector Anopheles gambiae.Nature communications · 2026Article
- Cryptic sex in Leishmania depends on SPO11 paralogs.PLoS pathogens · 2026Article
- Modifying meiotic recombination by targeting chromatin regulators to crossover hotspots inScience advances · 2026Article
- Cryptic Sex inbioRxiv : the preprint server for biology · 2026Article
- Loss of E3 ligase HvST1 function substantially increases distal crossover frequency.The New phytologist · 2026Article
- The synaptonemal complex central element SCEP3 interlinks synapsis initiation and crossover formation in Arabidopsis thaliana.Nature plants · 2025Article
- Apomixis at high resolution: unravelling diplospory in Asteraceae.Journal of experimental botany · 2025Article
- Spo11: from topoisomerase VI to meiotic recombination initiator.Biochemical Society transactions · 2025Review
- Article
- dCas9-SPO11-1 locally stimulates meiotic recombination in rice.Frontiers in plant science · 2025Article
- The plant early recombinosome: a high security complex to break DNA during meiosis.Plant reproduction · 2024Review
- Phylogenetic distribution of DNA topoisomerase VI and its distinction from SPO11.NAR genomics and bioinformatics · 2024Article
- Meiotic double-strand break repair DNA synthesis tracts in Arabidopsis thaliana.PLoS genetics · 2024Article
- Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana.Nucleic acids research · 2024Article
- Article
- Molecular mechanisms and regulation of recombination frequency and distribution in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Engineering apomixis in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Tracing the evolution of the plant meiotic molecular machinery.Plant reproduction · 2023Article
- The meiotic topoisomerase VI B subunit (MTOPVIB) is essential for meiotic DNA double-strand break formation in barley (Hordeum vulgare L.).Plant reproduction · 2023Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SPO11, a homolog of the subunit A of the archaebacterial topoisomerase VI, is essential for double-strand break (DSB)-induced initiation of meiotic recombination. In contrast with single homologs in animals and yeasts, three homologs are present in Arabidopsis thaliana and other higher plants. Whereas At SPO11-3 is involved in somatic endoreduplication, At SPO11-1 and, as recently shown, At SPO11-2 are essential for the initiation of meiotic recombination. Further defining the role of At SPO11-2, we were able to demonstrate that it is required for proper chromosome segregation, as its loss resulted in aneuploidy in the surviving progeny. The double mutant spo11-1 spo11-2 does not differ phenotypically from the single mutants, indicating that both proteins are required for the same step. Contrary to the observations for the At rad51-1 single mutant, the combination of spo11-2 and rad51-1 did not lead to chromosome fragmentation, indicating that SPO11-2, like SPO11-1, is required for DSB induction. As the meiotic phenotype of both single SPO11 mutants can be reversed by complementation using the full-length genes but not the same constructs mutated in their respective catalytically active Tyr, both proteins seem to participate directly in the DNA breakage reaction. The active involvement of two SPO11 homologs for DSB formation reveals a striking difference between plants and other eukaryotes in meiosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.