Evidence map›Paper›PMID 17965211›Full record

ArticleApplied and environmental microbiology2007

Density gradient enrichment of Escherichia coli conditional msbA mutants.

William T Doerrler

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Gadolinium Complexes as Contrast Agent for Cellular NMR Spectroscopy.International journal of molecular sciences · 2020
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

William T DoerrlerDepartment of Biological Sciences, Louisiana State University, 202 Life Sciences Building, Baton Rouge, LA 70803, USA. wdoerr@lsu.edu

Funding

BIOSYNTHESIS AND FUNCTION OF LIPOPOLYSACCHARIDESR01GM051310 · NIGMS · DUKE UNIVERSITY · PI ZHOU, PEI · 1994 to 2013
$8.1M
THE ROLE OF MSBA IN LPS SECRETION BY E. COLIF32AI010613 · NIAID · DUKE UNIVERSITY · PI DOERRLER, WILLIAM T · 2000 to 2003
$125k
NIAID NIH HHS 1 F32 AI-10613-01NIAID NIH HHS F32 AI010613NIGMS NIH HHS GM-51310NIGMS NIH HHS R01 GM051310
6 · The paper itself

Abstract

Insight into the mechanism of lipid transport to the outer membrane of gram-negative bacteria has been hampered by the lack of an effective genetic screen for defective mutants. This work demonstrates an enrichment of conditional mutants defective in lipopolysaccharide export by Ludox density gradient centrifugation and selection for detergent resistance. New temperature-sensitive mutants with lipid export defects were isolated with single missense mutations in msbA. The results demonstrate the power of this approach for the study of lipid export in Escherichia coli.

Indexed as

ATP-Binding Cassette TransportersBacterial ProteinsCentrifugation, Density GradientEscherichia coliLipopolysaccharidesMutant ProteinsMutation, MissenseSilicon DioxideATP-Binding Cassette TransportersBacterial ProteinsLipopolysaccharidesLudox AMMsbA protein, BacteriaMutant ProteinsSilicon Dioxide

Identifiers

PMID17965211
PMCPMC2168148

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.