ArticleApplied and environmental microbiology2008
Development and implementation of a single-chain Fv antibody for specific detection of Bacillus anthracis spores.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Fully human recombinant antibodies against EphA2 from a multi-tumor patient immune library suitable for tumor-targeted therapy.Bioengineered · 2021Article
- Challenges in Detection of Serum Oncoprotein: Relevance to Breast Cancer Diagnostics.Breast cancer (Dove Medical Press) · 2021Review
- Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy.Frontiers in cellular and infection microbiology · 2021Review
- Infectious disease antibodies for biomedical applications: A mini review of immune antibody phage library repertoire.International journal of biological macromolecules · 2020Review
- Development of an Antigen Detection ELISA for Bancroftian Filariasis UsingThe American journal of tropical medicine and hygiene · 2019Article
- The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions.Journal of visualized experiments : JoVE · 2018Article
- Recombinant antibodies for diagnostics and therapy against pathogens and toxins generated by phage display.Proteomics. Clinical applications · 2016Review
- Recombinant antibodies and their use in biosensors.Analytical and bioanalytical chemistry · 2012Review
- Construction of a single-chain variable-fragment antibody against the superantigen Staphylococcal enterotoxin B.Applied and environmental microbiology · 2010Article
- Generation of Fab fragment-like molecular recognition proteins against staphylococcal enterotoxin B by phage display technology.Clinical and vaccine immunology : CVI · 2010Article
- Detection of B. anthracis spores and vegetative cells with the same monoclonal antibodies.PloS one · 2009Article
- Antibody Fragments as Probe in Biosensor Development.Sensors (Basel, Switzerland) · 2008Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A single-chain Fv (scFv) antibody was developed and applied for efficient and specific detection of Bacillus anthracis spores. The antibody was isolated from a phage display library prepared from spleens of mice immunized with a water-soluble extract of the outer membrane of the B. anthracis spore (exosporium). The library (7 x 10(6) PFU) was biopanned against live, native B. anthracis ATCC delta14185 spores suspended in solution, resulting in the isolation of a unique soluble scFv antibody. The antibody was affinity purified and its affinity constant (3 x 10(8) +/- 1 x 10(8) M(-1)) determined via flow cytometry (FCM). Preliminary characterization of scFv specificity indicated that the scFv antibody does not cross-react with representatives of some phylogenetically related Bacillus spores. The potential use of scFv antibodies in detection platforms was demonstrated by the successful application of the soluble purified scFv antibody in enzyme-linked immunosorbent assays, immunofluorescence assays, and FCM.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.