ArticleApplied and environmental microbiology2008
Orthopoxvirus detection in environmental specimens during suspected bioterror attacks: inhibitory influences of common household products.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Development of an amplicon-based sequencing approach in response to the global emergence of mpox.PLoS biology · 2023Article
- Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus.medRxiv : the preprint server for health sciences · 2023Article
- Ongoing monkeypox virus outbreak, Portugal, 29 April to 23 May 2022.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2022Article
- Review
- Necrotic ulcerated lesion in a young boy caused by cowpox virus infection.Case reports in dermatology · 2011Article
- Detection limit of negative staining electron microscopy for the diagnosis of bioterrorism-related micro-organisms.Journal of applied microbiology · 2010Article
- Real-time PCR assay for detection of a new simulant for poxvirus biothreat agents.Applied and environmental microbiology · 2009Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
After terrorists attacked the United States in 2001, the appearance of letters and other objects containing powdery substances with unknown potentials for biological threat focused attention on the speed, sensitivity, and reliability of diagnostic methods. This study summarizes the abilities and limitations of real-time PCR, electron microscopy (EM), and virus isolation when used to detect potential bioweapons. In particular, we investigated the inhibitory influences of different common household products present in environmental specimens on PCR yield, EM detection, and virus isolation. We used vaccinia virus as a model for orthopoxviruses by spiking it into specimens. In the second part of the study, we describe modifications of diagnostic methods to overcome inhibitory effects. A variety of PCR amplification enhancers, DNA extraction protocols, and applications of internal controls were evaluated to improve diagnostic simplicity, speed, and reliability. As a result, we strongly recommend using at least two different frontline techniques in parallel, e.g., EM and PCR. A positive result obtained by any one of these techniques should be followed by a biological method to confirm the putative diagnosis. Confirmatory methods include virus isolation followed by an agent-specific immunofluorescence assay to confirm the presence of replication-competent particles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.