ArticlePlant physiology2007
Newly formed vacuoles in root meristems of barley and pea seedlings have characteristics of both protein storage and lytic vacuoles.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 71 citations in OpenAlex.
- Peptide AEDL Activates Metabolism and Autophagy in Root Cells ofInternational journal of molecular sciences · 2025Article
- The clues offered by SNAREs on the vacuoles of plants and animals.Frontiers in plant science · 2025Review
- The journey of cardosin A in young Arabidopsis seedlings leads to evidence of a Golgi-independent pathway to the protein storage vacuole.Frontiers in plant science · 2023Article
- Correlation of vacuole morphology with stomatal lineage development by whole-cell electron tomography.Plant physiology · 2022Article
- Coping with Abiotic Stress in Plants-An Endomembrane Trafficking Perspective.Plants (Basel, Switzerland) · 2022Review
- The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress.International journal of molecular sciences · 2021Article
- Arabidopsis tonoplast intrinsic protein and vacuolar HProtoplasma · 2019Article
- Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds.Bio-protocol · 2018Article
- Protein Storage Vacuoles Originate from Remodeled Preexisting Vacuoles inPlant physiology · 2018Article
- Annexins as Overlooked Regulators of Membrane Trafficking in Plant Cells.International journal of molecular sciences · 2017Review
- The relationship between vacuolation and initiation of PCD in rice (Oryza sativa) aleurone cells.Scientific reports · 2017Article
- Knock-Down of a Tonoplast Localized Low-Affinity Nitrate TransporterFrontiers in plant science · 2016Article
- Interactions between plant endomembrane systems and the actin cytoskeleton.Frontiers in plant science · 2015Review
- Delivering of proteins to the plant vacuole--an update.International journal of molecular sciences · 2014Review
- Plant vacuole morphology and vacuolar trafficking.Frontiers in plant science · 2014Review
- Following vegetative to embryonic cellular changes in leaves of Arabidopsis overexpressing LEAFY COTYLEDON2.Plant physiology · 2013Article
- Reprogramming cells to study vacuolar development.Frontiers in plant science · 2013Article
- Article
- Storage globulins pass through the Golgi apparatus and multivesicular bodies in the absence of dense vesicle formation during early stages of cotyledon development in mung bean.Journal of experimental botany · 2012Article
- A small molecule inhibitor partitions two distinct pathways for trafficking of tonoplast intrinsic proteins in Arabidopsis.PloS one · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant cells are considered to possess functionally different types of vacuoles in the same cell. One of the papers cited in support of this concept reported that protein storage and lytic vacuoles in root tips of barley (Hordeum vulgare) and pea (Pisum sativum) seedlings were initially separate compartments that later fused to form a central vacuole during cell elongation. We have reinvestigated the situation in these two roots using immunogold electron microscopy as well as immunofluorescence microscopy of histological sections. Using antisera generated against the whole protein of alpha-tonoplast intrinsic protein (TIP) as well as specific C-terminal TIP peptide antisera against alpha-, gamma-, and delta-TIP, together with antisera against the storage proteins barley lectin and pea legumin and vicilin, we were unable to obtain evidence for separate vacuole populations. Instead, our observations point to the formation of a single type of vacuole in cells differentiating both proximally and distally from the root meristem. This is a hybrid-type vacuole containing storage proteins and having both alpha- and gamma-TIPs, but not delta-TIP, in its tonoplast. As cells differentiate toward the zone of elongation, their vacuoles are characterized by increasing amounts of gamma-TIP and decreasing amounts of alpha-TIP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.