ArticlePlant physiology2007
Building blocks for plant gene assembly.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 128 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
128 citing papers in PubMed.
- Protocols for Root Developmental Studies in White Lupin.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Dexamethasone-inducible LhGR/pOp system: simple and flexible spatiotemporal control of gene expression.Planta · 2026Review
- Metal-sensing properties of the disordered loop from the Arabidopsis metal transceptor IRT1.The Biochemical journal · 2025Article
- GERMIN3 regulates tuber initiation and axillary bud activation by facilitating plasmodesmatal gating.The Plant journal : for cell and molecular biology · 2025Article
- Regulation and function of a polarly localized lignin barrier in the exodermis.Nature plants · 2025Article
- Evolution of the basic helix-loop-helix transcription factor SPATULA and its role in gynoecium development.Annals of botany · 2024Article
- Mapping the membrane orientation of auxin homeostasis regulators PIN5 and PIN8 in Arabidopsis thaliana root cells reveals their divergent topology.Plant methods · 2024Article
- ZmPILS6 is an auxin efflux carrier required for maize root morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- SUPPRESSOR OF MAX2 1-LIKE (SMXL) homologs are MAX2-dependent repressors of Physcomitrium patens growth.The Plant cell · 2024Article
- A suberized exodermis is required for tomato drought tolerance.Nature plants · 2024Article
- Transcriptional and Metabolic Profiling ofMetabolites · 2023Article
- Phosphorylation of ADAPTOR PROTEIN-2 μ-adaptin by ADAPTOR-ASSOCIATED KINASE1 regulates the tropic growth of Arabidopsis roots.The Plant cell · 2023Article
- From qualitative to quantitative: the state of the art and challenges for plant synthetic biology.Quantitative biology (Beijing, China) · 2023Review
- ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana.Nature communications · 2023Article
- Dynamic GOLVEN-ROOT GROWTH FACTOR 1 INSENSITIVE signaling in the root cap mediates root gravitropism.Plant physiology · 2023Article
- ABCB-mediated shootward auxin transport feeds into the root clock.EMBO reports · 2023Article
- Fast-track transformation and genome editing in Brachypodium distachyon.Plant methods · 2023Article
- Plant lineage-specific PIKMIN1 drives APC/CCCS52A2 E3-ligase activity-dependent cell division.Plant physiology · 2023Article
- Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform.Genome biology · 2023Article
- Efficient assembly of long DNA fragments and multiple genes with improved nickase-based cloning and Cre/loxP recombination.Plant biotechnology journal · 2022Article
68 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The MultiSite Gateway cloning system, based on site-specific recombination, enables the assembly of multiple DNA fragments in predefined order, orientation, and frame register. To streamline the construction of recombinant genes for functional analysis in plants, we have built a collection of 36 reference Gateway entry clones carrying promoters, terminators, and reporter genes, as well as elements of the LhG4/LhGR two-component system. This collection obeys simple engineering rules. The genetic elements (parts) are designed in a standard format. They are interchangeable, fully documented, and can be combined at will according to the desired output. We also took advantage of the MultiSite Gateway recombination sites to create vectors in which two or three genes can be cloned simultaneously in separate expression cassettes. To illustrate the flexibility of these core resources for the construction of a wide variety of plant transformation vectors, we generated various transgenes encoding fluorescent proteins and tested their activity in plant cells. The structure and sequence of all described plasmids are accessible online at http://www.psb.ugent.be/gateway/. All accessions can be requested via the same Web site.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.