ArticleJournal of bacteriology2008
Roles of ring-hydroxylating dioxygenases in styrene and benzene catabolism in Rhodococcus jostii RHA1.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 87 citations in OpenAlex.
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- Microbial and molecular approaches for PFAS transformation in soils: prospects and limitations.RSC advances · 2026Review
- Surfing in the storm: how Paraburkholderia xenovorans thrives under stress during biodegradation of toxic aromatic compounds and other stressors.FEMS microbiology reviews · 2025Review
- A mycofactocin-associated dehydrogenase is essential for ethylene glycol metabolism by Rhodococcus jostii RHA1.Applied microbiology and biotechnology · 2024Article
- The long-chain flavodoxin FldX1 improves the biodegradation of 4-hydroxyphenylacetate and 3-hydroxyphenylacetate and counteracts the oxidative stress associated to aromatic catabolism in Paraburkholderia xenovorans.Biological research · 2024Article
- Bottom-up construction and screening of algae-bacteria consortia for pollutant biodegradation.Frontiers in microbiology · 2024Article
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- Insights into plastic biodegradation: community composition and functional capabilities of the superworm (Microbial genomics · 2022Article
- Characterization of the GlutathioneMicrobiology spectrum · 2021Article
- Potential for and Distribution of Enzymatic Biodegradation of Polystyrene by Environmental Microorganisms.Materials (Basel, Switzerland) · 2021Article
- BTEX biodegradation by Bacillus amyloliquefaciens subsp. plantarum W1 and its proposed BTEX biodegradation pathways.Scientific reports · 2020Article
- Comparative Genomics of theMicroorganisms · 2020Article
- Analysis of the biodegradative and adaptive potential of the novel polychlorinated biphenyl degraderMicrobial genomics · 2020Article
- Characterization and Transcriptional Regulation ofMicroorganisms · 2019Article
- Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation ofBiotechnology for biofuels · 2019Article
- Article
- Genome-based analysis for the identification of genes involved in o-xylene degradation in Rhodococcus opacus R7.BMC genomics · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteomics and targeted gene disruption were used to investigate the catabolism of benzene, styrene, biphenyl, and ethylbenzene in Rhodococcus jostii RHA1, a well-studied soil bacterium whose potent polychlorinated biphenyl (PCB)-transforming properties are partly due to the presence of the related Bph and Etb pathways. Of 151 identified proteins, 22 Bph/Etb proteins were among the most abundant in biphenyl-, ethylbenzene-, benzene-, and styrene-grown cells. Cells grown on biphenyl, ethylbenzene, or benzene contained both Bph and Etb enzymes and at least two sets of lower Bph pathway enzymes. By contrast, styrene-grown cells contained no Etb enzymes and only one set of lower Bph pathway enzymes. Gene disruption established that biphenyl dioxygenase (BPDO) was essential for growth of RHA1 on benzene or styrene but that ethylbenzene dioxygenase (EBDO) was not required for growth on any of the tested substrates. Moreover, whole-cell assays of the delta bphAa and etbAa1::cmrA etbAa2::aphII mutants demonstrated that while both dioxygenases preferentially transformed biphenyl, only BPDO transformed styrene. Deletion of pcaL of the beta-ketoadipate pathway disrupted growth on benzene but not other substrates. Thus, styrene and benzene are degraded via meta- and ortho-cleavage, respectively. Finally, catalases were more abundant during growth on nonpolar aromatic compounds than on aromatic acids. This suggests that the relaxed specificities of BPDO and EBDO that enable RHA1 to grow on a range of compounds come at the cost of increased uncoupling during the latter's initial transformation. The stress response may augment RHA1's ability to degrade PCBs and other pollutants that induce similar uncoupling.
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